Cargando…
Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758)
Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appea...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878368/ https://www.ncbi.nlm.nih.gov/pubmed/27231653 http://dx.doi.org/10.7717/peerj.2019 |
_version_ | 1782433556824326144 |
---|---|
author | Ewers-Saucedo, Christine Zardus, John D. Wares, John P. |
author_facet | Ewers-Saucedo, Christine Zardus, John D. Wares, John P. |
author_sort | Ewers-Saucedo, Christine |
collection | PubMed |
description | Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appears to be a host generalist, and has an unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers, such as microsatellite markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies on C. testudinaria. |
format | Online Article Text |
id | pubmed-4878368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48783682016-05-26 Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) Ewers-Saucedo, Christine Zardus, John D. Wares, John P. PeerJ Ecology Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appears to be a host generalist, and has an unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers, such as microsatellite markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies on C. testudinaria. PeerJ Inc. 2016-05-17 /pmc/articles/PMC4878368/ /pubmed/27231653 http://dx.doi.org/10.7717/peerj.2019 Text en © 2016 Ewers-Saucedo et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Ecology Ewers-Saucedo, Christine Zardus, John D. Wares, John P. Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) |
title | Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) |
title_full | Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) |
title_fullStr | Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) |
title_full_unstemmed | Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) |
title_short | Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758) |
title_sort | microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle chelonibia testudinaria (linnaeus, 1758) |
topic | Ecology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878368/ https://www.ncbi.nlm.nih.gov/pubmed/27231653 http://dx.doi.org/10.7717/peerj.2019 |
work_keys_str_mv | AT ewerssaucedochristine microsatellitelocidiscoveryfromnextgenerationsequencingdataandlocicharacterizationintheepizoicbarnaclechelonibiatestudinarialinnaeus1758 AT zardusjohnd microsatellitelocidiscoveryfromnextgenerationsequencingdataandlocicharacterizationintheepizoicbarnaclechelonibiatestudinarialinnaeus1758 AT waresjohnp microsatellitelocidiscoveryfromnextgenerationsequencingdataandlocicharacterizationintheepizoicbarnaclechelonibiatestudinarialinnaeus1758 |