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Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals

Synucleinopathies and abnormalities in the nerves of the enteric nervous system are hypothesized to be involved in age-associated motility disorders. The aim of the present study was to investigate the expression of various antigens, including α-synuclein (Syn) and its post-translational modified fo...

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Autores principales: XUAN, QI, ZHANG, YU-XIAN, LIU, DIAN-GANG, CHAN, PIU, XU, SHENG-LI, CUI, YE-QING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878562/
https://www.ncbi.nlm.nih.gov/pubmed/27109489
http://dx.doi.org/10.3892/mmr.2016.5166
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author XUAN, QI
ZHANG, YU-XIAN
LIU, DIAN-GANG
CHAN, PIU
XU, SHENG-LI
CUI, YE-QING
author_facet XUAN, QI
ZHANG, YU-XIAN
LIU, DIAN-GANG
CHAN, PIU
XU, SHENG-LI
CUI, YE-QING
author_sort XUAN, QI
collection PubMed
description Synucleinopathies and abnormalities in the nerves of the enteric nervous system are hypothesized to be involved in age-associated motility disorders. The aim of the present study was to investigate the expression of various antigens, including α-synuclein (Syn) and its post-translational modified forms, in the human colon at various ages. In addition, the study aimed to correlate the expression of Syn with neurodegeneration. Immunohistochemistry was used to detect the expression of neurofilament (NF), Syn, as well as its nitrated (N) form in the healthy colonic tissue of 12 young (34.08±5.12 years), 10 middle-aged (51.80±3.52 years), and 11 elderly (75.82±7.70 years) individuals. To the best of our knowledge, the current study is the first to demonstrate the presence of N-Syn in the colonic tissue. N-Syn was identified in the upper layer of the mucosa and submucosa layer. Furthermore, Syn (wild-type) was present in the mucosa and submucosa. The number of NF-positive neurons in the submucosal layer declined significantly with age (P<0.01). In addition, Syn and N-Syn significantly increased during aging (P<0.01). Furthermore, a negative correlation was identified between neuron number and synucleinopathies, indicating the abnormal accumulation of both wild-type Syn and N-Syn in the mucosa, submucosa, muscle layer and myenteric plexus. The present study demonstrates that the Syn pathology may be linked to colic neuronal degeneration during normal aging, and this link may cause functional deficits.
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spelling pubmed-48785622016-05-25 Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals XUAN, QI ZHANG, YU-XIAN LIU, DIAN-GANG CHAN, PIU XU, SHENG-LI CUI, YE-QING Mol Med Rep Articles Synucleinopathies and abnormalities in the nerves of the enteric nervous system are hypothesized to be involved in age-associated motility disorders. The aim of the present study was to investigate the expression of various antigens, including α-synuclein (Syn) and its post-translational modified forms, in the human colon at various ages. In addition, the study aimed to correlate the expression of Syn with neurodegeneration. Immunohistochemistry was used to detect the expression of neurofilament (NF), Syn, as well as its nitrated (N) form in the healthy colonic tissue of 12 young (34.08±5.12 years), 10 middle-aged (51.80±3.52 years), and 11 elderly (75.82±7.70 years) individuals. To the best of our knowledge, the current study is the first to demonstrate the presence of N-Syn in the colonic tissue. N-Syn was identified in the upper layer of the mucosa and submucosa layer. Furthermore, Syn (wild-type) was present in the mucosa and submucosa. The number of NF-positive neurons in the submucosal layer declined significantly with age (P<0.01). In addition, Syn and N-Syn significantly increased during aging (P<0.01). Furthermore, a negative correlation was identified between neuron number and synucleinopathies, indicating the abnormal accumulation of both wild-type Syn and N-Syn in the mucosa, submucosa, muscle layer and myenteric plexus. The present study demonstrates that the Syn pathology may be linked to colic neuronal degeneration during normal aging, and this link may cause functional deficits. D.A. Spandidos 2016-06 2016-04-22 /pmc/articles/PMC4878562/ /pubmed/27109489 http://dx.doi.org/10.3892/mmr.2016.5166 Text en Copyright: © Xuan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
XUAN, QI
ZHANG, YU-XIAN
LIU, DIAN-GANG
CHAN, PIU
XU, SHENG-LI
CUI, YE-QING
Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
title Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
title_full Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
title_fullStr Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
title_full_unstemmed Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
title_short Post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
title_sort post-translational modifications of α-synuclein contribute to neurodegeneration in the colon of elderly individuals
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878562/
https://www.ncbi.nlm.nih.gov/pubmed/27109489
http://dx.doi.org/10.3892/mmr.2016.5166
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