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Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome

BACKGROUND: Jian-Pi-Zhi-Dong Decoction (JPZDD) is a dedicated treatment of Tourette syndrome (TS). The balance of neurotransmitters in the cortico-striato-pallido-thalamo-cortical network is crucial to the occurrence of TS and related to its severity. This study evaluated the effect of JPZDD on glut...

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Autores principales: Zhang, Wen, Wei, Li, Yu, Wenjing, Cui, Xia, Liu, Xiaofang, Wang, Qian, Wang, Sumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878666/
https://www.ncbi.nlm.nih.gov/pubmed/27279743
http://dx.doi.org/10.2147/NDT.S106330
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author Zhang, Wen
Wei, Li
Yu, Wenjing
Cui, Xia
Liu, Xiaofang
Wang, Qian
Wang, Sumei
author_facet Zhang, Wen
Wei, Li
Yu, Wenjing
Cui, Xia
Liu, Xiaofang
Wang, Qian
Wang, Sumei
author_sort Zhang, Wen
collection PubMed
description BACKGROUND: Jian-Pi-Zhi-Dong Decoction (JPZDD) is a dedicated treatment of Tourette syndrome (TS). The balance of neurotransmitters in the cortico-striato-pallido-thalamo-cortical network is crucial to the occurrence of TS and related to its severity. This study evaluated the effect of JPZDD on glutamate (Glu) and γ-aminobutyric acid (GABA) and their receptors in a TS rat model. MATERIALS AND METHODS: Rats were divided into four groups (n=12 each). TS was induced in three of the groups by injecting them with 3,3′-iminodipropionitrile for 7 consecutive days. Two model groups were treated with tiapride (Tia) or JPZDD, while the control and the remaining model group were gavaged with saline. Behavior was assessed by stereotypic score and autonomic activity. Striatal Glu and GABA contents were detected using microdialysis. Expressions of N-methyl-D-aspartate receptor 1 and GABA(A) receptor (GABA(A)R) were observed using Western blot and real-time polymerase chain reaction. RESULTS: Tia and JPZDD groups had decreased stereotypy compared with model rats; however, the JPZDD group showed a larger decrease in stereotypy than the Tia group at a 4-week time point. In a spontaneous activity test, the total distance of the JPZDD and Tia groups was significantly decreased compared with the model group. The Glu levels of the model group were higher than the control group and decreased with Tia or JPZDD treatment. The GABA level was higher in the model group than the control group. Expressions of GABA(A)R protein in the model group were higher than in the control group. Treatment with Tia or JPZDD reduced the expression of GABA(A)R protein. In the case of the mRNA expression, only Tia reduced the expression of N-methyl-D-aspartate receptor 1, compared with the model group. CONCLUSION: JPZDD could alleviate impairments in behavior and dysfunctional signaling by downregulating GABA(A)R in the striatum. We suggest that this acts to maintain the balance of Glu and GABA.
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spelling pubmed-48786662016-06-08 Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome Zhang, Wen Wei, Li Yu, Wenjing Cui, Xia Liu, Xiaofang Wang, Qian Wang, Sumei Neuropsychiatr Dis Treat Original Research BACKGROUND: Jian-Pi-Zhi-Dong Decoction (JPZDD) is a dedicated treatment of Tourette syndrome (TS). The balance of neurotransmitters in the cortico-striato-pallido-thalamo-cortical network is crucial to the occurrence of TS and related to its severity. This study evaluated the effect of JPZDD on glutamate (Glu) and γ-aminobutyric acid (GABA) and their receptors in a TS rat model. MATERIALS AND METHODS: Rats were divided into four groups (n=12 each). TS was induced in three of the groups by injecting them with 3,3′-iminodipropionitrile for 7 consecutive days. Two model groups were treated with tiapride (Tia) or JPZDD, while the control and the remaining model group were gavaged with saline. Behavior was assessed by stereotypic score and autonomic activity. Striatal Glu and GABA contents were detected using microdialysis. Expressions of N-methyl-D-aspartate receptor 1 and GABA(A) receptor (GABA(A)R) were observed using Western blot and real-time polymerase chain reaction. RESULTS: Tia and JPZDD groups had decreased stereotypy compared with model rats; however, the JPZDD group showed a larger decrease in stereotypy than the Tia group at a 4-week time point. In a spontaneous activity test, the total distance of the JPZDD and Tia groups was significantly decreased compared with the model group. The Glu levels of the model group were higher than the control group and decreased with Tia or JPZDD treatment. The GABA level was higher in the model group than the control group. Expressions of GABA(A)R protein in the model group were higher than in the control group. Treatment with Tia or JPZDD reduced the expression of GABA(A)R protein. In the case of the mRNA expression, only Tia reduced the expression of N-methyl-D-aspartate receptor 1, compared with the model group. CONCLUSION: JPZDD could alleviate impairments in behavior and dysfunctional signaling by downregulating GABA(A)R in the striatum. We suggest that this acts to maintain the balance of Glu and GABA. Dove Medical Press 2016-05-19 /pmc/articles/PMC4878666/ /pubmed/27279743 http://dx.doi.org/10.2147/NDT.S106330 Text en © 2016 Zhang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhang, Wen
Wei, Li
Yu, Wenjing
Cui, Xia
Liu, Xiaofang
Wang, Qian
Wang, Sumei
Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome
title Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome
title_full Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome
title_fullStr Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome
title_full_unstemmed Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome
title_short Effect of Jian-Pi-Zhi-Dong Decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of Tourette syndrome
title_sort effect of jian-pi-zhi-dong decoction on striatal glutamate and γ-aminobutyric acid levels detected using microdialysis in a rat model of tourette syndrome
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878666/
https://www.ncbi.nlm.nih.gov/pubmed/27279743
http://dx.doi.org/10.2147/NDT.S106330
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