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De novo transcriptome assembly of Sorghum bicolor variety Taejin

Sorghum (Sorghum bicolor), also known as great millet, is one of the most popular cultivated grass species in the world. Sorghum is frequently consumed as food for humans and animals as well as used for ethanol production. In this study, we conducted de novo transcriptome assembly for sorghum variet...

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Autores principales: Jo, Yeonhwa, Lian, Sen, Cho, Jin Kyong, Choi, Hoseong, Kim, Sang-Min, Kim, Sun-Lim, Lee, Bong Choon, Cho, Won Kyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878842/
https://www.ncbi.nlm.nih.gov/pubmed/27257604
http://dx.doi.org/10.1016/j.gdata.2016.05.002
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author Jo, Yeonhwa
Lian, Sen
Cho, Jin Kyong
Choi, Hoseong
Kim, Sang-Min
Kim, Sun-Lim
Lee, Bong Choon
Cho, Won Kyong
author_facet Jo, Yeonhwa
Lian, Sen
Cho, Jin Kyong
Choi, Hoseong
Kim, Sang-Min
Kim, Sun-Lim
Lee, Bong Choon
Cho, Won Kyong
author_sort Jo, Yeonhwa
collection PubMed
description Sorghum (Sorghum bicolor), also known as great millet, is one of the most popular cultivated grass species in the world. Sorghum is frequently consumed as food for humans and animals as well as used for ethanol production. In this study, we conducted de novo transcriptome assembly for sorghum variety Taejin by next-generation sequencing, obtaining 8.748 GB of raw data. The raw data in this study can be available in NCBI SRA database with accession number of SRX1715644. Using the Trinity program, we identified 222,161 transcripts from sorghum variety Taejin. We further predicted coding regions within the assembled transcripts by the TransDecoder program, resulting in a total of 148,531 proteins. We carried out BLASTP against the Swiss-Prot protein sequence database to annotate the functions of the identified proteins. To our knowledge, this is the first transcriptome data for a sorghum variety derived from Korea, and it can be usefully applied to the generation of genetic markers.
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spelling pubmed-48788422016-06-02 De novo transcriptome assembly of Sorghum bicolor variety Taejin Jo, Yeonhwa Lian, Sen Cho, Jin Kyong Choi, Hoseong Kim, Sang-Min Kim, Sun-Lim Lee, Bong Choon Cho, Won Kyong Genom Data Data in Brief Article Sorghum (Sorghum bicolor), also known as great millet, is one of the most popular cultivated grass species in the world. Sorghum is frequently consumed as food for humans and animals as well as used for ethanol production. In this study, we conducted de novo transcriptome assembly for sorghum variety Taejin by next-generation sequencing, obtaining 8.748 GB of raw data. The raw data in this study can be available in NCBI SRA database with accession number of SRX1715644. Using the Trinity program, we identified 222,161 transcripts from sorghum variety Taejin. We further predicted coding regions within the assembled transcripts by the TransDecoder program, resulting in a total of 148,531 proteins. We carried out BLASTP against the Swiss-Prot protein sequence database to annotate the functions of the identified proteins. To our knowledge, this is the first transcriptome data for a sorghum variety derived from Korea, and it can be usefully applied to the generation of genetic markers. Elsevier 2016-05-05 /pmc/articles/PMC4878842/ /pubmed/27257604 http://dx.doi.org/10.1016/j.gdata.2016.05.002 Text en © 2016 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data in Brief Article
Jo, Yeonhwa
Lian, Sen
Cho, Jin Kyong
Choi, Hoseong
Kim, Sang-Min
Kim, Sun-Lim
Lee, Bong Choon
Cho, Won Kyong
De novo transcriptome assembly of Sorghum bicolor variety Taejin
title De novo transcriptome assembly of Sorghum bicolor variety Taejin
title_full De novo transcriptome assembly of Sorghum bicolor variety Taejin
title_fullStr De novo transcriptome assembly of Sorghum bicolor variety Taejin
title_full_unstemmed De novo transcriptome assembly of Sorghum bicolor variety Taejin
title_short De novo transcriptome assembly of Sorghum bicolor variety Taejin
title_sort de novo transcriptome assembly of sorghum bicolor variety taejin
topic Data in Brief Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878842/
https://www.ncbi.nlm.nih.gov/pubmed/27257604
http://dx.doi.org/10.1016/j.gdata.2016.05.002
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