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Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome
PURPOSE: To investigate the properties of angiogenin (ANG) as a potential tool for the diagnosis and grading of dry eye syndrome (DES) by analyzing tear protein profiles. METHODS: Tear samples were collected with capillary tubes from 52 DES patients and 29 normal individuals as controls. Tear protei...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Ophthalmological Society
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878976/ https://www.ncbi.nlm.nih.gov/pubmed/27247515 http://dx.doi.org/10.3341/kjo.2016.30.3.163 |
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author | Kim, Won Soo Wee, Sung Wook Lee, Seung Hoon Kim, Jae Chan |
author_facet | Kim, Won Soo Wee, Sung Wook Lee, Seung Hoon Kim, Jae Chan |
author_sort | Kim, Won Soo |
collection | PubMed |
description | PURPOSE: To investigate the properties of angiogenin (ANG) as a potential tool for the diagnosis and grading of dry eye syndrome (DES) by analyzing tear protein profiles. METHODS: Tear samples were collected with capillary tubes from 52 DES patients and 29 normal individuals as controls. Tear protein profiles were analyzed with an immunodot blot assay as a screening test. To confirm that the tear ANG levels were in inverse proportion to the disease severity grade, the ANG and lactoferrin (LF) tear contents of normal controls and DES patients were compared in an enzyme-linked immunosorbent assay. RESULTS: In the immunodot blot assay, the ANG area was lower in patients with grades 3 and 4 DES than in normal controls. The areas of basic fibroblast growth factor, transforming growth factor β2, and interleukin 10 were significantly greater than those of normal controls only in grade 4 DES patients, but these proteins were not linearly correlated with dry eye severity. Upon enzyme-linked immunosorbent assay analysis, the mean concentrations of ANG and LF decreased significantly as dry eye severity increased, except between grades 1 and 2. In addition, the ratios of ANG and LF to total tear proteins were correlated significantly with DES severity. CONCLUSIONS: ANG level was significantly lower in DES patients than in normal controls, and was significantly correlated with the worsening severity of DES, except between grades 1 and 2, as was LF. Therefore, ANG may be a useful measure of DES severity through proteomic analysis. |
format | Online Article Text |
id | pubmed-4878976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Korean Ophthalmological Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-48789762016-06-01 Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome Kim, Won Soo Wee, Sung Wook Lee, Seung Hoon Kim, Jae Chan Korean J Ophthalmol Original Article PURPOSE: To investigate the properties of angiogenin (ANG) as a potential tool for the diagnosis and grading of dry eye syndrome (DES) by analyzing tear protein profiles. METHODS: Tear samples were collected with capillary tubes from 52 DES patients and 29 normal individuals as controls. Tear protein profiles were analyzed with an immunodot blot assay as a screening test. To confirm that the tear ANG levels were in inverse proportion to the disease severity grade, the ANG and lactoferrin (LF) tear contents of normal controls and DES patients were compared in an enzyme-linked immunosorbent assay. RESULTS: In the immunodot blot assay, the ANG area was lower in patients with grades 3 and 4 DES than in normal controls. The areas of basic fibroblast growth factor, transforming growth factor β2, and interleukin 10 were significantly greater than those of normal controls only in grade 4 DES patients, but these proteins were not linearly correlated with dry eye severity. Upon enzyme-linked immunosorbent assay analysis, the mean concentrations of ANG and LF decreased significantly as dry eye severity increased, except between grades 1 and 2. In addition, the ratios of ANG and LF to total tear proteins were correlated significantly with DES severity. CONCLUSIONS: ANG level was significantly lower in DES patients than in normal controls, and was significantly correlated with the worsening severity of DES, except between grades 1 and 2, as was LF. Therefore, ANG may be a useful measure of DES severity through proteomic analysis. The Korean Ophthalmological Society 2016-06 2016-05-18 /pmc/articles/PMC4878976/ /pubmed/27247515 http://dx.doi.org/10.3341/kjo.2016.30.3.163 Text en © 2016 The Korean Ophthalmological Society http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Won Soo Wee, Sung Wook Lee, Seung Hoon Kim, Jae Chan Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome |
title | Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome |
title_full | Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome |
title_fullStr | Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome |
title_full_unstemmed | Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome |
title_short | Angiogenin for the Diagnosis and Grading of Dry Eye Syndrome |
title_sort | angiogenin for the diagnosis and grading of dry eye syndrome |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4878976/ https://www.ncbi.nlm.nih.gov/pubmed/27247515 http://dx.doi.org/10.3341/kjo.2016.30.3.163 |
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