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Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans

Chamaecyparis obtusa (C. obtusa) is known to have antimicrobial effects and has been used as a medicinal plant and in forest bathing. This study aimed to evaluate the anticariogenic activity of essential oil of C. obtusa on Streptococcus mutans, which is one of the most important bacterial causes of...

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Autores principales: Kim, Eun-Hee, Kang, Sun-Young, Park, Bog-Im, Kim, Young-Hoi, Lee, Young-Rae, Hoe, Jin-Hee, Choi, Na-Young, Ra, Ji-Young, An, So-Youn, You, Yong-Ouk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879247/
https://www.ncbi.nlm.nih.gov/pubmed/27293453
http://dx.doi.org/10.1155/2016/2396404
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author Kim, Eun-Hee
Kang, Sun-Young
Park, Bog-Im
Kim, Young-Hoi
Lee, Young-Rae
Hoe, Jin-Hee
Choi, Na-Young
Ra, Ji-Young
An, So-Youn
You, Yong-Ouk
author_facet Kim, Eun-Hee
Kang, Sun-Young
Park, Bog-Im
Kim, Young-Hoi
Lee, Young-Rae
Hoe, Jin-Hee
Choi, Na-Young
Ra, Ji-Young
An, So-Youn
You, Yong-Ouk
author_sort Kim, Eun-Hee
collection PubMed
description Chamaecyparis obtusa (C. obtusa) is known to have antimicrobial effects and has been used as a medicinal plant and in forest bathing. This study aimed to evaluate the anticariogenic activity of essential oil of C. obtusa on Streptococcus mutans, which is one of the most important bacterial causes of dental caries and dental biofilm formation. Essential oil from C. obtusa was extracted, and its effect on bacterial growth, acid production, and biofilm formation was evaluated. C. obtusa essential oil exhibited concentration-dependent inhibition of bacterial growth over 0.025 mg/mL, with 99% inhibition at a concentration of 0.2 mg/mL. The bacterial biofilm formation and acid production were also significantly inhibited at the concentration greater than 0.025 mg/mL. The result of LIVE/DEAD® BacLight™ Bacterial Viability Kit showed a concentration-dependent bactericidal effect on S. mutans and almost all bacteria were dead over 0.8 mg/mL. Real-time PCR analysis showed that gene expression of some virulence factors such as brpA, gbpB, gtfC, and gtfD was also inhibited. In GC and GC-MS analysis, the major components were found to be α-terpinene (40.60%), bornyl acetate (12.45%), α-pinene (11.38%), β-pinene (7.22%), β-phellandrene (3.45%), and α-terpinolene (3.40%). These results show that C. obtusa essential oil has anticariogenic effect on S. mutans.
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spelling pubmed-48792472016-06-12 Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans Kim, Eun-Hee Kang, Sun-Young Park, Bog-Im Kim, Young-Hoi Lee, Young-Rae Hoe, Jin-Hee Choi, Na-Young Ra, Ji-Young An, So-Youn You, Yong-Ouk Evid Based Complement Alternat Med Research Article Chamaecyparis obtusa (C. obtusa) is known to have antimicrobial effects and has been used as a medicinal plant and in forest bathing. This study aimed to evaluate the anticariogenic activity of essential oil of C. obtusa on Streptococcus mutans, which is one of the most important bacterial causes of dental caries and dental biofilm formation. Essential oil from C. obtusa was extracted, and its effect on bacterial growth, acid production, and biofilm formation was evaluated. C. obtusa essential oil exhibited concentration-dependent inhibition of bacterial growth over 0.025 mg/mL, with 99% inhibition at a concentration of 0.2 mg/mL. The bacterial biofilm formation and acid production were also significantly inhibited at the concentration greater than 0.025 mg/mL. The result of LIVE/DEAD® BacLight™ Bacterial Viability Kit showed a concentration-dependent bactericidal effect on S. mutans and almost all bacteria were dead over 0.8 mg/mL. Real-time PCR analysis showed that gene expression of some virulence factors such as brpA, gbpB, gtfC, and gtfD was also inhibited. In GC and GC-MS analysis, the major components were found to be α-terpinene (40.60%), bornyl acetate (12.45%), α-pinene (11.38%), β-pinene (7.22%), β-phellandrene (3.45%), and α-terpinolene (3.40%). These results show that C. obtusa essential oil has anticariogenic effect on S. mutans. Hindawi Publishing Corporation 2016 2016-05-11 /pmc/articles/PMC4879247/ /pubmed/27293453 http://dx.doi.org/10.1155/2016/2396404 Text en Copyright © 2016 Eun-Hee Kim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kim, Eun-Hee
Kang, Sun-Young
Park, Bog-Im
Kim, Young-Hoi
Lee, Young-Rae
Hoe, Jin-Hee
Choi, Na-Young
Ra, Ji-Young
An, So-Youn
You, Yong-Ouk
Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans
title Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans
title_full Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans
title_fullStr Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans
title_full_unstemmed Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans
title_short Chamaecyparis obtusa Suppresses Virulence Genes in Streptococcus mutans
title_sort chamaecyparis obtusa suppresses virulence genes in streptococcus mutans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879247/
https://www.ncbi.nlm.nih.gov/pubmed/27293453
http://dx.doi.org/10.1155/2016/2396404
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