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Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications
Synthetic biology is characterized by the development of novel and powerful DNA fabrication methods and by the application of engineering principles to biology. The current study describes Terminator Operon Reporter (TOR), a new gene assembly technology based on the conditional activation of a repor...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879669/ https://www.ncbi.nlm.nih.gov/pubmed/27220405 http://dx.doi.org/10.1038/srep26572 |
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author | Zampini, Massimiliano Mur, Luis A. J. Rees Stevens, Pauline Pachebat, Justin A. Newbold, C. James Hayes, Finbarr Kingston-Smith, Alison |
author_facet | Zampini, Massimiliano Mur, Luis A. J. Rees Stevens, Pauline Pachebat, Justin A. Newbold, C. James Hayes, Finbarr Kingston-Smith, Alison |
author_sort | Zampini, Massimiliano |
collection | PubMed |
description | Synthetic biology is characterized by the development of novel and powerful DNA fabrication methods and by the application of engineering principles to biology. The current study describes Terminator Operon Reporter (TOR), a new gene assembly technology based on the conditional activation of a reporter gene in response to sequence errors occurring at the assembly stage of the synthetic element. These errors are monitored by a transcription terminator that is placed between the synthetic gene and reporter gene. Switching of this terminator between active and inactive states dictates the transcription status of the downstream reporter gene to provide a rapid and facile readout of the accuracy of synthetic assembly. Designed specifically and uniquely for the synthesis of protein coding genes in bacteria, TOR allows the rapid and cost-effective fabrication of synthetic constructs by employing oligonucleotides at the most basic purification level (desalted) and without the need for costly and time-consuming post-synthesis correction methods. Thus, TOR streamlines gene assembly approaches, which are central to the future development of synthetic biology. |
format | Online Article Text |
id | pubmed-4879669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48796692016-06-07 Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications Zampini, Massimiliano Mur, Luis A. J. Rees Stevens, Pauline Pachebat, Justin A. Newbold, C. James Hayes, Finbarr Kingston-Smith, Alison Sci Rep Article Synthetic biology is characterized by the development of novel and powerful DNA fabrication methods and by the application of engineering principles to biology. The current study describes Terminator Operon Reporter (TOR), a new gene assembly technology based on the conditional activation of a reporter gene in response to sequence errors occurring at the assembly stage of the synthetic element. These errors are monitored by a transcription terminator that is placed between the synthetic gene and reporter gene. Switching of this terminator between active and inactive states dictates the transcription status of the downstream reporter gene to provide a rapid and facile readout of the accuracy of synthetic assembly. Designed specifically and uniquely for the synthesis of protein coding genes in bacteria, TOR allows the rapid and cost-effective fabrication of synthetic constructs by employing oligonucleotides at the most basic purification level (desalted) and without the need for costly and time-consuming post-synthesis correction methods. Thus, TOR streamlines gene assembly approaches, which are central to the future development of synthetic biology. Nature Publishing Group 2016-05-25 /pmc/articles/PMC4879669/ /pubmed/27220405 http://dx.doi.org/10.1038/srep26572 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zampini, Massimiliano Mur, Luis A. J. Rees Stevens, Pauline Pachebat, Justin A. Newbold, C. James Hayes, Finbarr Kingston-Smith, Alison Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
title | Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
title_full | Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
title_fullStr | Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
title_full_unstemmed | Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
title_short | Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
title_sort | terminator operon reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879669/ https://www.ncbi.nlm.nih.gov/pubmed/27220405 http://dx.doi.org/10.1038/srep26572 |
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