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Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells

Here we report a powerful method that facilitates the transport of biologically active materials across the cell wall barrier in plant cells. Positively charged delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) with a 0.5‒2 nm thickness and 30‒60 nm diameter exhibit a high ads...

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Autores principales: Bao, Wenlong, Wang, Junya, Wang, Qiang, O’Hare, Dermot, Wan, Yinglang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879670/
https://www.ncbi.nlm.nih.gov/pubmed/27221055
http://dx.doi.org/10.1038/srep26738
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author Bao, Wenlong
Wang, Junya
Wang, Qiang
O’Hare, Dermot
Wan, Yinglang
author_facet Bao, Wenlong
Wang, Junya
Wang, Qiang
O’Hare, Dermot
Wan, Yinglang
author_sort Bao, Wenlong
collection PubMed
description Here we report a powerful method that facilitates the transport of biologically active materials across the cell wall barrier in plant cells. Positively charged delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) with a 0.5‒2 nm thickness and 30‒60 nm diameter exhibit a high adsorptive capacity for negatively charged biomolecules, including fluorescent dyes such as tetramethyl rhodamine isothiocyanate (TRITC), fluorescein isothiocyanate isomer I(FITC) and DNA molecules, forming neutral LDH-nanosheet conjugates. These neutral conjugates can shuttle the bound fluorescent dye into the cytosol of intact plant cell very efficiently. Furthermore, typical inhibitors of endocytosis and low temperature incubation did not prevent LDH-lactate-NS internalization, suggesting that LDH-lactate-NS penetrated the plasma membrane via non-endocytic pathways, which will widen the applicability to a variety of plant cells. Moreover, the absence of unwanted side effects in our cytological studies, and the nuclear localization of ssDNA-FITC suggest that nano-LDHs have potential application as a novel gene carrier to plants.
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spelling pubmed-48796702016-06-07 Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells Bao, Wenlong Wang, Junya Wang, Qiang O’Hare, Dermot Wan, Yinglang Sci Rep Article Here we report a powerful method that facilitates the transport of biologically active materials across the cell wall barrier in plant cells. Positively charged delaminated layered double hydroxide lactate nanosheets (LDH-lactate-NS) with a 0.5‒2 nm thickness and 30‒60 nm diameter exhibit a high adsorptive capacity for negatively charged biomolecules, including fluorescent dyes such as tetramethyl rhodamine isothiocyanate (TRITC), fluorescein isothiocyanate isomer I(FITC) and DNA molecules, forming neutral LDH-nanosheet conjugates. These neutral conjugates can shuttle the bound fluorescent dye into the cytosol of intact plant cell very efficiently. Furthermore, typical inhibitors of endocytosis and low temperature incubation did not prevent LDH-lactate-NS internalization, suggesting that LDH-lactate-NS penetrated the plasma membrane via non-endocytic pathways, which will widen the applicability to a variety of plant cells. Moreover, the absence of unwanted side effects in our cytological studies, and the nuclear localization of ssDNA-FITC suggest that nano-LDHs have potential application as a novel gene carrier to plants. Nature Publishing Group 2016-05-25 /pmc/articles/PMC4879670/ /pubmed/27221055 http://dx.doi.org/10.1038/srep26738 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Bao, Wenlong
Wang, Junya
Wang, Qiang
O’Hare, Dermot
Wan, Yinglang
Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells
title Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells
title_full Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells
title_fullStr Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells
title_full_unstemmed Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells
title_short Layered Double Hydroxide Nanotransporter for Molecule Delivery to Intact Plant Cells
title_sort layered double hydroxide nanotransporter for molecule delivery to intact plant cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879670/
https://www.ncbi.nlm.nih.gov/pubmed/27221055
http://dx.doi.org/10.1038/srep26738
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