Cargando…
Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo
Small diameter arterial bypass grafts are considered as unmet clinical need since the current grafts have poor patency of 25% within 5 years. We have developed a 3D scaffold manufactured from natural and synthetic biodegradable polymers, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(𝜀...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879758/ https://www.ncbi.nlm.nih.gov/pubmed/27252652 http://dx.doi.org/10.3389/fphar.2016.00136 |
_version_ | 1782433720835244032 |
---|---|
author | Antonova, Larisa V. Seifalian, Alexander M. Kutikhin, Anton G. Sevostyanova, Victoria V. Krivkina, Evgeniya O. Mironov, Andrey V. Burago, Andrey Y. Velikanova, Elena A. Matveeva, Vera G. Glushkova, Tatiana V. Sergeeva, Evgeniya A. Vasyukov, Georgiy Y. Kudryavtseva, Yuliya A. Barbarash, Olga L. Barbarash, Leonid S. |
author_facet | Antonova, Larisa V. Seifalian, Alexander M. Kutikhin, Anton G. Sevostyanova, Victoria V. Krivkina, Evgeniya O. Mironov, Andrey V. Burago, Andrey Y. Velikanova, Elena A. Matveeva, Vera G. Glushkova, Tatiana V. Sergeeva, Evgeniya A. Vasyukov, Georgiy Y. Kudryavtseva, Yuliya A. Barbarash, Olga L. Barbarash, Leonid S. |
author_sort | Antonova, Larisa V. |
collection | PubMed |
description | Small diameter arterial bypass grafts are considered as unmet clinical need since the current grafts have poor patency of 25% within 5 years. We have developed a 3D scaffold manufactured from natural and synthetic biodegradable polymers, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(𝜀-caprolactone) (PCL), respectively. Further to improve the biophysical properties as well as endothelialisation, the grafts were covalently conjugated with arginine-glycine-aspartic acid (RGD) bioactive peptides. The biophysical properties as well as endothelialisation of PHBV/PCL and PCL 2 mm diameter bypass grafts were assessed with and without biofunctionalisation with RGD peptides in vitro and in vivo. Morphology of the grafts was assessed by scanning electron microscopy, whereas physico-mechanical properties were evaluated using a physiological circulating system equipped with a state of art ultrasound vascular wall tracking system. Endothelialisation of the grafts in vitro and in vivo were assessed using a cell viability assay and rat abdominal aorta replacement model, respectively. The biofunctionalisation with RGD bioactive peptides decreased mean fiber diameter and mean pore area in PHBV/PCL grafts; however, this was not the case for PCL grafts. Both PHBV/PCL and PCL grafts with RGD peptides had lower durability compared to those without; these durability values were similar to those of internal mammary artery. Modification of PHBV/PCL and PCL grafts with RGD peptides increased endothelial cell viability in vitro by a factor of eight and enhanced the formation of an endothelial cell monolayer in vivo 1 month postimplantation. In conclusion, PHBV/PCL small-caliber graft can be a suitable 3D scaffold for the development of a tissue engineering arterial bypass graft. |
format | Online Article Text |
id | pubmed-4879758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48797582016-06-01 Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo Antonova, Larisa V. Seifalian, Alexander M. Kutikhin, Anton G. Sevostyanova, Victoria V. Krivkina, Evgeniya O. Mironov, Andrey V. Burago, Andrey Y. Velikanova, Elena A. Matveeva, Vera G. Glushkova, Tatiana V. Sergeeva, Evgeniya A. Vasyukov, Georgiy Y. Kudryavtseva, Yuliya A. Barbarash, Olga L. Barbarash, Leonid S. Front Pharmacol Pharmacology Small diameter arterial bypass grafts are considered as unmet clinical need since the current grafts have poor patency of 25% within 5 years. We have developed a 3D scaffold manufactured from natural and synthetic biodegradable polymers, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) and poly(𝜀-caprolactone) (PCL), respectively. Further to improve the biophysical properties as well as endothelialisation, the grafts were covalently conjugated with arginine-glycine-aspartic acid (RGD) bioactive peptides. The biophysical properties as well as endothelialisation of PHBV/PCL and PCL 2 mm diameter bypass grafts were assessed with and without biofunctionalisation with RGD peptides in vitro and in vivo. Morphology of the grafts was assessed by scanning electron microscopy, whereas physico-mechanical properties were evaluated using a physiological circulating system equipped with a state of art ultrasound vascular wall tracking system. Endothelialisation of the grafts in vitro and in vivo were assessed using a cell viability assay and rat abdominal aorta replacement model, respectively. The biofunctionalisation with RGD bioactive peptides decreased mean fiber diameter and mean pore area in PHBV/PCL grafts; however, this was not the case for PCL grafts. Both PHBV/PCL and PCL grafts with RGD peptides had lower durability compared to those without; these durability values were similar to those of internal mammary artery. Modification of PHBV/PCL and PCL grafts with RGD peptides increased endothelial cell viability in vitro by a factor of eight and enhanced the formation of an endothelial cell monolayer in vivo 1 month postimplantation. In conclusion, PHBV/PCL small-caliber graft can be a suitable 3D scaffold for the development of a tissue engineering arterial bypass graft. Frontiers Media S.A. 2016-05-25 /pmc/articles/PMC4879758/ /pubmed/27252652 http://dx.doi.org/10.3389/fphar.2016.00136 Text en Copyright © 2016 Antonova, Seifalian, Kutikhin, Sevostyanova, Krivkina, Mironov, Burago, Velikanova, Matveeva, Glushkova, Sergeeva, Vasyukov, Kudryavtseva, Barbarash and Barbarash. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Antonova, Larisa V. Seifalian, Alexander M. Kutikhin, Anton G. Sevostyanova, Victoria V. Krivkina, Evgeniya O. Mironov, Andrey V. Burago, Andrey Y. Velikanova, Elena A. Matveeva, Vera G. Glushkova, Tatiana V. Sergeeva, Evgeniya A. Vasyukov, Georgiy Y. Kudryavtseva, Yuliya A. Barbarash, Olga L. Barbarash, Leonid S. Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo |
title | Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo |
title_full | Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo |
title_fullStr | Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo |
title_full_unstemmed | Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo |
title_short | Bioabsorbable Bypass Grafts Biofunctionalised with RGD Have Enhanced Biophysical Properties and Endothelialisation Tested In vivo |
title_sort | bioabsorbable bypass grafts biofunctionalised with rgd have enhanced biophysical properties and endothelialisation tested in vivo |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879758/ https://www.ncbi.nlm.nih.gov/pubmed/27252652 http://dx.doi.org/10.3389/fphar.2016.00136 |
work_keys_str_mv | AT antonovalarisav bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT seifalianalexanderm bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT kutikhinantong bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT sevostyanovavictoriav bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT krivkinaevgeniyao bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT mironovandreyv bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT buragoandreyy bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT velikanovaelenaa bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT matveevaverag bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT glushkovatatianav bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT sergeevaevgeniyaa bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT vasyukovgeorgiyy bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT kudryavtsevayuliyaa bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT barbarasholgal bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo AT barbarashleonids bioabsorbablebypassgraftsbiofunctionalisedwithrgdhaveenhancedbiophysicalpropertiesandendothelialisationtestedinvivo |