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Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN

Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of t...

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Autores principales: SHARBATKHORI, Mitra, TANZIFI, Asal, ROSTAMI, Sima, ROSTAMI, Masoomeh, HARANDI, Majid FASIHI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879995/
https://www.ncbi.nlm.nih.gov/pubmed/27253740
http://dx.doi.org/10.1590/S1678-9946201658038
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author SHARBATKHORI, Mitra
TANZIFI, Asal
ROSTAMI, Sima
ROSTAMI, Masoomeh
HARANDI, Majid FASIHI
author_facet SHARBATKHORI, Mitra
TANZIFI, Asal
ROSTAMI, Sima
ROSTAMI, Masoomeh
HARANDI, Majid FASIHI
author_sort SHARBATKHORI, Mitra
collection PubMed
description Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. Seventy E. granulosus isolates were collected from animals in slaughterhouses: 18 isolates from sheep, 40 from cattle, nine from camels, two from buffaloes and one from a goat, along with four human isolates (formalin-fixed, paraffin-embedded tissues) from CE patients of provincial hospitals. All isolates were successfully analysed by PCR amplification and sequencing. The sequence analysis found four E. granulosus genotypes among the 74 CE isolates: G1 (78.3%), G2 (2.7%), G3 (15%) and G6 (4%). The G1-G3 complex genotype was found in all of the sheep, goat, cattle and buffalo isolates. Among the nine camel isolates, the frequency of G1-G3 and G6 genotypes were 66.7% and 33.3%, respectively. All four human CE isolates belonged to E. granulosus sensu stricto. This study reports the first occurrence of the G2 genotype in cattle from Iran and confirms the previously reported G3 genotype in camels in the same country.
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spelling pubmed-48799952016-06-03 Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN SHARBATKHORI, Mitra TANZIFI, Asal ROSTAMI, Sima ROSTAMI, Masoomeh HARANDI, Majid FASIHI Rev Inst Med Trop Sao Paulo Original Article Cystic echinococcosis (CE) is a globally parasitic zoonosis caused by larval stages of Echinococcus granulosus. This study investigated E. granulosus genotypes isolated from livestock and humans in the Golestan province, northern Iran, southeast of the Caspian sea, using partial sequencing data of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. Seventy E. granulosus isolates were collected from animals in slaughterhouses: 18 isolates from sheep, 40 from cattle, nine from camels, two from buffaloes and one from a goat, along with four human isolates (formalin-fixed, paraffin-embedded tissues) from CE patients of provincial hospitals. All isolates were successfully analysed by PCR amplification and sequencing. The sequence analysis found four E. granulosus genotypes among the 74 CE isolates: G1 (78.3%), G2 (2.7%), G3 (15%) and G6 (4%). The G1-G3 complex genotype was found in all of the sheep, goat, cattle and buffalo isolates. Among the nine camel isolates, the frequency of G1-G3 and G6 genotypes were 66.7% and 33.3%, respectively. All four human CE isolates belonged to E. granulosus sensu stricto. This study reports the first occurrence of the G2 genotype in cattle from Iran and confirms the previously reported G3 genotype in camels in the same country. Instituto de Medicina Tropical 2016-05-24 /pmc/articles/PMC4879995/ /pubmed/27253740 http://dx.doi.org/10.1590/S1678-9946201658038 Text en http://creativecommons.org/licenses/by-nc/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
SHARBATKHORI, Mitra
TANZIFI, Asal
ROSTAMI, Sima
ROSTAMI, Masoomeh
HARANDI, Majid FASIHI
Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_full Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_fullStr Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_full_unstemmed Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_short Echinococcus granulosus sensu lato GENOTYPES IN DOMESTIC LIVESTOCK AND HUMANS IN GOLESTAN PROVINCE, IRAN
title_sort echinococcus granulosus sensu lato genotypes in domestic livestock and humans in golestan province, iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4879995/
https://www.ncbi.nlm.nih.gov/pubmed/27253740
http://dx.doi.org/10.1590/S1678-9946201658038
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