Cargando…

Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet

Currently, knowledge about the impact of long-term intake of high resistant starch diet on pig hindgut microbiota and metabolite profile is limited. In this study, a combination of the pyrosequencing and the mass spectrometry (MS)-based metabolomics techniques were used to investigate the effects of...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Yue, Su, Yong, Zhu, Weiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880592/
https://www.ncbi.nlm.nih.gov/pubmed/27303373
http://dx.doi.org/10.3389/fmicb.2016.00779
_version_ 1782433815267901440
author Sun, Yue
Su, Yong
Zhu, Weiyun
author_facet Sun, Yue
Su, Yong
Zhu, Weiyun
author_sort Sun, Yue
collection PubMed
description Currently, knowledge about the impact of long-term intake of high resistant starch diet on pig hindgut microbiota and metabolite profile is limited. In this study, a combination of the pyrosequencing and the mass spectrometry (MS)-based metabolomics techniques were used to investigate the effects of a raw potato starch (RPS, high in resistant starch) diet on microbial composition and microbial metabolites in the hindgut of pig. The results showed that Coprococcus, Ruminococcus, and Turicibacter increased significantly, while Sarcina and Clostridium decreased in relative abundances in the hindgut of pigs fed RPS. The metabolimic analysis revealed that RPS significantly affected starch and sucrose metabolites, amino acid turnover or protein biosynthesis, lipid metabolites, glycolysis, the pentose phosphate pathway, inositol phosphate metabolism, and nucleotide metabolism. Furthermore, a Pearson's correlation analysis showed that Ruminococcus and Coprococcus were positively correlated with glucose-6-phosphate, maltose, arachidonic acid, 9, 12-octadecadienoic acid, oleic acid, phosphate, but negatively correlated with α-aminobutyric acid. However, the correlation of Clostridium and Sarcina with these compounds was in the opposite direction. The results suggest that RPS not only alters the composition of the gut microbial community but also modulates the metabolic pathway of microbial metabolism, which may further affect the hindgut health of the host.
format Online
Article
Text
id pubmed-4880592
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-48805922016-06-14 Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet Sun, Yue Su, Yong Zhu, Weiyun Front Microbiol Microbiology Currently, knowledge about the impact of long-term intake of high resistant starch diet on pig hindgut microbiota and metabolite profile is limited. In this study, a combination of the pyrosequencing and the mass spectrometry (MS)-based metabolomics techniques were used to investigate the effects of a raw potato starch (RPS, high in resistant starch) diet on microbial composition and microbial metabolites in the hindgut of pig. The results showed that Coprococcus, Ruminococcus, and Turicibacter increased significantly, while Sarcina and Clostridium decreased in relative abundances in the hindgut of pigs fed RPS. The metabolimic analysis revealed that RPS significantly affected starch and sucrose metabolites, amino acid turnover or protein biosynthesis, lipid metabolites, glycolysis, the pentose phosphate pathway, inositol phosphate metabolism, and nucleotide metabolism. Furthermore, a Pearson's correlation analysis showed that Ruminococcus and Coprococcus were positively correlated with glucose-6-phosphate, maltose, arachidonic acid, 9, 12-octadecadienoic acid, oleic acid, phosphate, but negatively correlated with α-aminobutyric acid. However, the correlation of Clostridium and Sarcina with these compounds was in the opposite direction. The results suggest that RPS not only alters the composition of the gut microbial community but also modulates the metabolic pathway of microbial metabolism, which may further affect the hindgut health of the host. Frontiers Media S.A. 2016-05-26 /pmc/articles/PMC4880592/ /pubmed/27303373 http://dx.doi.org/10.3389/fmicb.2016.00779 Text en Copyright © 2016 Sun, Su and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Sun, Yue
Su, Yong
Zhu, Weiyun
Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
title Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
title_full Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
title_fullStr Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
title_full_unstemmed Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
title_short Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
title_sort microbiome-metabolome responses in the cecum and colon of pig to a high resistant starch diet
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880592/
https://www.ncbi.nlm.nih.gov/pubmed/27303373
http://dx.doi.org/10.3389/fmicb.2016.00779
work_keys_str_mv AT sunyue microbiomemetabolomeresponsesinthececumandcolonofpigtoahighresistantstarchdiet
AT suyong microbiomemetabolomeresponsesinthececumandcolonofpigtoahighresistantstarchdiet
AT zhuweiyun microbiomemetabolomeresponsesinthececumandcolonofpigtoahighresistantstarchdiet