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Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet
Currently, knowledge about the impact of long-term intake of high resistant starch diet on pig hindgut microbiota and metabolite profile is limited. In this study, a combination of the pyrosequencing and the mass spectrometry (MS)-based metabolomics techniques were used to investigate the effects of...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880592/ https://www.ncbi.nlm.nih.gov/pubmed/27303373 http://dx.doi.org/10.3389/fmicb.2016.00779 |
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author | Sun, Yue Su, Yong Zhu, Weiyun |
author_facet | Sun, Yue Su, Yong Zhu, Weiyun |
author_sort | Sun, Yue |
collection | PubMed |
description | Currently, knowledge about the impact of long-term intake of high resistant starch diet on pig hindgut microbiota and metabolite profile is limited. In this study, a combination of the pyrosequencing and the mass spectrometry (MS)-based metabolomics techniques were used to investigate the effects of a raw potato starch (RPS, high in resistant starch) diet on microbial composition and microbial metabolites in the hindgut of pig. The results showed that Coprococcus, Ruminococcus, and Turicibacter increased significantly, while Sarcina and Clostridium decreased in relative abundances in the hindgut of pigs fed RPS. The metabolimic analysis revealed that RPS significantly affected starch and sucrose metabolites, amino acid turnover or protein biosynthesis, lipid metabolites, glycolysis, the pentose phosphate pathway, inositol phosphate metabolism, and nucleotide metabolism. Furthermore, a Pearson's correlation analysis showed that Ruminococcus and Coprococcus were positively correlated with glucose-6-phosphate, maltose, arachidonic acid, 9, 12-octadecadienoic acid, oleic acid, phosphate, but negatively correlated with α-aminobutyric acid. However, the correlation of Clostridium and Sarcina with these compounds was in the opposite direction. The results suggest that RPS not only alters the composition of the gut microbial community but also modulates the metabolic pathway of microbial metabolism, which may further affect the hindgut health of the host. |
format | Online Article Text |
id | pubmed-4880592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-48805922016-06-14 Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet Sun, Yue Su, Yong Zhu, Weiyun Front Microbiol Microbiology Currently, knowledge about the impact of long-term intake of high resistant starch diet on pig hindgut microbiota and metabolite profile is limited. In this study, a combination of the pyrosequencing and the mass spectrometry (MS)-based metabolomics techniques were used to investigate the effects of a raw potato starch (RPS, high in resistant starch) diet on microbial composition and microbial metabolites in the hindgut of pig. The results showed that Coprococcus, Ruminococcus, and Turicibacter increased significantly, while Sarcina and Clostridium decreased in relative abundances in the hindgut of pigs fed RPS. The metabolimic analysis revealed that RPS significantly affected starch and sucrose metabolites, amino acid turnover or protein biosynthesis, lipid metabolites, glycolysis, the pentose phosphate pathway, inositol phosphate metabolism, and nucleotide metabolism. Furthermore, a Pearson's correlation analysis showed that Ruminococcus and Coprococcus were positively correlated with glucose-6-phosphate, maltose, arachidonic acid, 9, 12-octadecadienoic acid, oleic acid, phosphate, but negatively correlated with α-aminobutyric acid. However, the correlation of Clostridium and Sarcina with these compounds was in the opposite direction. The results suggest that RPS not only alters the composition of the gut microbial community but also modulates the metabolic pathway of microbial metabolism, which may further affect the hindgut health of the host. Frontiers Media S.A. 2016-05-26 /pmc/articles/PMC4880592/ /pubmed/27303373 http://dx.doi.org/10.3389/fmicb.2016.00779 Text en Copyright © 2016 Sun, Su and Zhu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Sun, Yue Su, Yong Zhu, Weiyun Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet |
title | Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet |
title_full | Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet |
title_fullStr | Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet |
title_full_unstemmed | Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet |
title_short | Microbiome-Metabolome Responses in the Cecum and Colon of Pig to a High Resistant Starch Diet |
title_sort | microbiome-metabolome responses in the cecum and colon of pig to a high resistant starch diet |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880592/ https://www.ncbi.nlm.nih.gov/pubmed/27303373 http://dx.doi.org/10.3389/fmicb.2016.00779 |
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