Cargando…
Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle
Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin dise...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880690/ https://www.ncbi.nlm.nih.gov/pubmed/27294125 http://dx.doi.org/10.1155/2016/6257984 |
_version_ | 1782433832497053696 |
---|---|
author | Şevik, Murat Avci, Oğuzhan Doğan, Müge İnce, Ömer Barış |
author_facet | Şevik, Murat Avci, Oğuzhan Doğan, Müge İnce, Ömer Barış |
author_sort | Şevik, Murat |
collection | PubMed |
description | Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection. |
format | Online Article Text |
id | pubmed-4880690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48806902016-06-12 Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle Şevik, Murat Avci, Oğuzhan Doğan, Müge İnce, Ömer Barış Biomed Res Int Research Article Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection. Hindawi Publishing Corporation 2016 2016-05-12 /pmc/articles/PMC4880690/ /pubmed/27294125 http://dx.doi.org/10.1155/2016/6257984 Text en Copyright © 2016 Murat Şevik et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Şevik, Murat Avci, Oğuzhan Doğan, Müge İnce, Ömer Barış Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle |
title | Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle |
title_full | Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle |
title_fullStr | Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle |
title_full_unstemmed | Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle |
title_short | Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle |
title_sort | serum biochemistry of lumpy skin disease virus-infected cattle |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880690/ https://www.ncbi.nlm.nih.gov/pubmed/27294125 http://dx.doi.org/10.1155/2016/6257984 |
work_keys_str_mv | AT sevikmurat serumbiochemistryoflumpyskindiseasevirusinfectedcattle AT avcioguzhan serumbiochemistryoflumpyskindiseasevirusinfectedcattle AT doganmuge serumbiochemistryoflumpyskindiseasevirusinfectedcattle AT inceomerbarıs serumbiochemistryoflumpyskindiseasevirusinfectedcattle |