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Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel
Heritable phenotypic differences between populations, caused by the selective effects of distinct environmental conditions, are of commonplace occurrence in nature. However, the actual genomic targets of this kind of selection are still poorly understood. We conducted a quantitative trait locus (QTL...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880927/ https://www.ncbi.nlm.nih.gov/pubmed/27226078 http://dx.doi.org/10.1038/srep26632 |
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author | Yang, Jing Guo, Baocheng Shikano, Takahito Liu, Xiaolin Merilä, Juha |
author_facet | Yang, Jing Guo, Baocheng Shikano, Takahito Liu, Xiaolin Merilä, Juha |
author_sort | Yang, Jing |
collection | PubMed |
description | Heritable phenotypic differences between populations, caused by the selective effects of distinct environmental conditions, are of commonplace occurrence in nature. However, the actual genomic targets of this kind of selection are still poorly understood. We conducted a quantitative trait locus (QTL) mapping study to identify genomic regions responsible for morphometric differentiation between genetically and phenotypically divergent marine and freshwater nine-spined stickleback (Pungitius pungitius) populations. Using a dense panel of SNP-markers obtained by restriction site associated DNA sequencing of an F(2) recombinant cross, we found 22 QTL that explained 3.5–12.9% of phenotypic variance in the traits under investigation. We detected one fairly large-effect (PVE = 9.6%) QTL for caudal peduncle length–a trait with a well-established adaptive function showing clear differentiation among marine and freshwater populations. We also identified two large-effect QTL for lateral plate numbers, which are different from the lateral plate QTL reported in earlier studies of this and related species. Hence, apart from identifying several large-effect QTL in shape traits showing adaptive differentiation in response to different environmental conditions, the results suggest intra- and interspecific heterogeneity in the genomic basis of lateral plate number variation. |
format | Online Article Text |
id | pubmed-4880927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48809272016-06-07 Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel Yang, Jing Guo, Baocheng Shikano, Takahito Liu, Xiaolin Merilä, Juha Sci Rep Article Heritable phenotypic differences between populations, caused by the selective effects of distinct environmental conditions, are of commonplace occurrence in nature. However, the actual genomic targets of this kind of selection are still poorly understood. We conducted a quantitative trait locus (QTL) mapping study to identify genomic regions responsible for morphometric differentiation between genetically and phenotypically divergent marine and freshwater nine-spined stickleback (Pungitius pungitius) populations. Using a dense panel of SNP-markers obtained by restriction site associated DNA sequencing of an F(2) recombinant cross, we found 22 QTL that explained 3.5–12.9% of phenotypic variance in the traits under investigation. We detected one fairly large-effect (PVE = 9.6%) QTL for caudal peduncle length–a trait with a well-established adaptive function showing clear differentiation among marine and freshwater populations. We also identified two large-effect QTL for lateral plate numbers, which are different from the lateral plate QTL reported in earlier studies of this and related species. Hence, apart from identifying several large-effect QTL in shape traits showing adaptive differentiation in response to different environmental conditions, the results suggest intra- and interspecific heterogeneity in the genomic basis of lateral plate number variation. Nature Publishing Group 2016-05-26 /pmc/articles/PMC4880927/ /pubmed/27226078 http://dx.doi.org/10.1038/srep26632 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Yang, Jing Guo, Baocheng Shikano, Takahito Liu, Xiaolin Merilä, Juha Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel |
title | Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel |
title_full | Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel |
title_fullStr | Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel |
title_full_unstemmed | Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel |
title_short | Quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density SNP-panel |
title_sort | quantitative trait locus analysis of body shape divergence in nine-spined sticklebacks based on high-density snp-panel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4880927/ https://www.ncbi.nlm.nih.gov/pubmed/27226078 http://dx.doi.org/10.1038/srep26632 |
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