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Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats
The aim of the present study was to investigate the effect of C. papaya L. leaf extract (CPLE) on pancreatic islets in streptozotocin (STZ)-induced diabetic rats, as well as on cultured normal pancreatic cells with STZ in the medium. CPLE (3–125 mg/Kg) was administered orally for 20 days, while a gr...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881071/ https://www.ncbi.nlm.nih.gov/pubmed/27128930 http://dx.doi.org/10.3390/ijerph13050446 |
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author | Miranda-Osorio, Pedro H. Castell-Rodríguez, Andrés E. Vargas-Mancilla, Juan Tovilla-Zárate, Carlos A. Ble-Castillo, Jorge L. Aguilar-Domínguez, Dora E. Juárez-Rojop, Isela E. Díaz-Zagoya, Juan C. |
author_facet | Miranda-Osorio, Pedro H. Castell-Rodríguez, Andrés E. Vargas-Mancilla, Juan Tovilla-Zárate, Carlos A. Ble-Castillo, Jorge L. Aguilar-Domínguez, Dora E. Juárez-Rojop, Isela E. Díaz-Zagoya, Juan C. |
author_sort | Miranda-Osorio, Pedro H. |
collection | PubMed |
description | The aim of the present study was to investigate the effect of C. papaya L. leaf extract (CPLE) on pancreatic islets in streptozotocin (STZ)-induced diabetic rats, as well as on cultured normal pancreatic cells with STZ in the medium. CPLE (3–125 mg/Kg) was administered orally for 20 days, while a group of diabetic rats received 5 IU/Kg/day of insulin. At the end of the treatment the rats were sacrificed. Blood was obtained to assess glucose and insulin levels. The pancreas was dissected to evaluate β cells by immunohistochemistry. In addition, normal pancreatic cells were cultured in a medium that included CPLE (3–12 mg). One half of the cultured cells received simultaneously CPLE and STZ (6 mg), while the other half received CPLE and five days later the STZ. After three days of incubation, insulin was assayed in the incubation medium. The CPLE administered to diabetic rats improved the fasting glycemia and preserved the number and structure of pancreatic islets. However, when CPLE was added to pancreatic cells in culture along with STZ, the insulin concentration was higher in comparison with the cells that only received STZ. In conclusion, the CPLE preserves the integrity of pancreatic islets, improves the basal insulin secretion and protects cultured cells from the adverse effects of STZ. |
format | Online Article Text |
id | pubmed-4881071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-48810712016-05-27 Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats Miranda-Osorio, Pedro H. Castell-Rodríguez, Andrés E. Vargas-Mancilla, Juan Tovilla-Zárate, Carlos A. Ble-Castillo, Jorge L. Aguilar-Domínguez, Dora E. Juárez-Rojop, Isela E. Díaz-Zagoya, Juan C. Int J Environ Res Public Health Article The aim of the present study was to investigate the effect of C. papaya L. leaf extract (CPLE) on pancreatic islets in streptozotocin (STZ)-induced diabetic rats, as well as on cultured normal pancreatic cells with STZ in the medium. CPLE (3–125 mg/Kg) was administered orally for 20 days, while a group of diabetic rats received 5 IU/Kg/day of insulin. At the end of the treatment the rats were sacrificed. Blood was obtained to assess glucose and insulin levels. The pancreas was dissected to evaluate β cells by immunohistochemistry. In addition, normal pancreatic cells were cultured in a medium that included CPLE (3–12 mg). One half of the cultured cells received simultaneously CPLE and STZ (6 mg), while the other half received CPLE and five days later the STZ. After three days of incubation, insulin was assayed in the incubation medium. The CPLE administered to diabetic rats improved the fasting glycemia and preserved the number and structure of pancreatic islets. However, when CPLE was added to pancreatic cells in culture along with STZ, the insulin concentration was higher in comparison with the cells that only received STZ. In conclusion, the CPLE preserves the integrity of pancreatic islets, improves the basal insulin secretion and protects cultured cells from the adverse effects of STZ. MDPI 2016-04-27 2016-05 /pmc/articles/PMC4881071/ /pubmed/27128930 http://dx.doi.org/10.3390/ijerph13050446 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Miranda-Osorio, Pedro H. Castell-Rodríguez, Andrés E. Vargas-Mancilla, Juan Tovilla-Zárate, Carlos A. Ble-Castillo, Jorge L. Aguilar-Domínguez, Dora E. Juárez-Rojop, Isela E. Díaz-Zagoya, Juan C. Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats |
title | Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats |
title_full | Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats |
title_fullStr | Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats |
title_full_unstemmed | Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats |
title_short | Protective Action of Carica papaya on β-Cells in Streptozotocin-Induced Diabetic Rats |
title_sort | protective action of carica papaya on β-cells in streptozotocin-induced diabetic rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881071/ https://www.ncbi.nlm.nih.gov/pubmed/27128930 http://dx.doi.org/10.3390/ijerph13050446 |
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