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Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches

Identification and quantitative monitoring of mutant BCR-ABL1 subclones displaying resistance to tyrosine kinase inhibitors (TKIs) have become important tasks in patients with Ph-positive leukemias. Different technologies have been established for patient screening. Various next-generation sequencin...

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Autores principales: Preuner, Sandra, Barna, Agnes, Frommlet, Florian, Czurda, Stefan, Konstantin, Byrgazov, Alikian, Mary, Machova Polakova, Katerina, Sacha, Tomasz, Richter, Johan, Lion, Thomas, Gabriel, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881468/
https://www.ncbi.nlm.nih.gov/pubmed/27136541
http://dx.doi.org/10.3390/ijms17050642
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author Preuner, Sandra
Barna, Agnes
Frommlet, Florian
Czurda, Stefan
Konstantin, Byrgazov
Alikian, Mary
Machova Polakova, Katerina
Sacha, Tomasz
Richter, Johan
Lion, Thomas
Gabriel, Christian
author_facet Preuner, Sandra
Barna, Agnes
Frommlet, Florian
Czurda, Stefan
Konstantin, Byrgazov
Alikian, Mary
Machova Polakova, Katerina
Sacha, Tomasz
Richter, Johan
Lion, Thomas
Gabriel, Christian
author_sort Preuner, Sandra
collection PubMed
description Identification and quantitative monitoring of mutant BCR-ABL1 subclones displaying resistance to tyrosine kinase inhibitors (TKIs) have become important tasks in patients with Ph-positive leukemias. Different technologies have been established for patient screening. Various next-generation sequencing (NGS) platforms facilitating sensitive detection and quantitative monitoring of mutations in the ABL1-kinase domain (KD) have been introduced recently, and are expected to become the preferred technology in the future. However, broad clinical implementation of NGS methods has been hampered by the limited accessibility at different centers and the current costs of analysis which may not be regarded as readily affordable for routine diagnostic monitoring. It is therefore of interest to determine whether NGS platforms can be adequately substituted by other methodological approaches. We have tested three different techniques including pyrosequencing, LD (ligation-dependent)-PCR and NGS in a series of peripheral blood specimens from chronic myeloid leukemia (CML) patients carrying single or multiple mutations in the BCR-ABL1 KD. The proliferation kinetics of mutant subclones in serial specimens obtained during the course of TKI-treatment revealed similar profiles via all technical approaches, but individual specimens showed statistically significant differences between NGS and the other methods tested. The observations indicate that different approaches to detection and quantification of mutant subclones may be applicable for the monitoring of clonal kinetics, but careful calibration of each method is required for accurate size assessment of mutant subclones at individual time points.
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spelling pubmed-48814682016-05-27 Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches Preuner, Sandra Barna, Agnes Frommlet, Florian Czurda, Stefan Konstantin, Byrgazov Alikian, Mary Machova Polakova, Katerina Sacha, Tomasz Richter, Johan Lion, Thomas Gabriel, Christian Int J Mol Sci Article Identification and quantitative monitoring of mutant BCR-ABL1 subclones displaying resistance to tyrosine kinase inhibitors (TKIs) have become important tasks in patients with Ph-positive leukemias. Different technologies have been established for patient screening. Various next-generation sequencing (NGS) platforms facilitating sensitive detection and quantitative monitoring of mutations in the ABL1-kinase domain (KD) have been introduced recently, and are expected to become the preferred technology in the future. However, broad clinical implementation of NGS methods has been hampered by the limited accessibility at different centers and the current costs of analysis which may not be regarded as readily affordable for routine diagnostic monitoring. It is therefore of interest to determine whether NGS platforms can be adequately substituted by other methodological approaches. We have tested three different techniques including pyrosequencing, LD (ligation-dependent)-PCR and NGS in a series of peripheral blood specimens from chronic myeloid leukemia (CML) patients carrying single or multiple mutations in the BCR-ABL1 KD. The proliferation kinetics of mutant subclones in serial specimens obtained during the course of TKI-treatment revealed similar profiles via all technical approaches, but individual specimens showed statistically significant differences between NGS and the other methods tested. The observations indicate that different approaches to detection and quantification of mutant subclones may be applicable for the monitoring of clonal kinetics, but careful calibration of each method is required for accurate size assessment of mutant subclones at individual time points. MDPI 2016-04-29 /pmc/articles/PMC4881468/ /pubmed/27136541 http://dx.doi.org/10.3390/ijms17050642 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Preuner, Sandra
Barna, Agnes
Frommlet, Florian
Czurda, Stefan
Konstantin, Byrgazov
Alikian, Mary
Machova Polakova, Katerina
Sacha, Tomasz
Richter, Johan
Lion, Thomas
Gabriel, Christian
Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches
title Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches
title_full Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches
title_fullStr Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches
title_full_unstemmed Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches
title_short Quantitative Analysis of Mutant Subclones in Chronic Myeloid Leukemia: Comparison of Different Methodological Approaches
title_sort quantitative analysis of mutant subclones in chronic myeloid leukemia: comparison of different methodological approaches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881468/
https://www.ncbi.nlm.nih.gov/pubmed/27136541
http://dx.doi.org/10.3390/ijms17050642
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