Cargando…

Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue

The molecular mechanism responsible for Ewing’s Sarcoma (ES) remains largely unknown. MicroRNAs (miRNAs), a class of small non-coding RNAs able to regulate gene expression, are deregulated in tumors and may serve as a tool for diagnosis and prediction. However, the status of miRNAs in ES has not yet...

Descripción completa

Detalles Bibliográficos
Autores principales: Parafioriti, Antonina, Bason, Caterina, Armiraglio, Elisabetta, Calciano, Lucia, Daolio, Primo Andrea, Berardocco, Martina, Di Bernardo, Andrea, Colosimo, Alessia, Luksch, Roberto, Berardi, Anna C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881482/
https://www.ncbi.nlm.nih.gov/pubmed/27144561
http://dx.doi.org/10.3390/ijms17050656
_version_ 1782433967748677632
author Parafioriti, Antonina
Bason, Caterina
Armiraglio, Elisabetta
Calciano, Lucia
Daolio, Primo Andrea
Berardocco, Martina
Di Bernardo, Andrea
Colosimo, Alessia
Luksch, Roberto
Berardi, Anna C.
author_facet Parafioriti, Antonina
Bason, Caterina
Armiraglio, Elisabetta
Calciano, Lucia
Daolio, Primo Andrea
Berardocco, Martina
Di Bernardo, Andrea
Colosimo, Alessia
Luksch, Roberto
Berardi, Anna C.
author_sort Parafioriti, Antonina
collection PubMed
description The molecular mechanism responsible for Ewing’s Sarcoma (ES) remains largely unknown. MicroRNAs (miRNAs), a class of small non-coding RNAs able to regulate gene expression, are deregulated in tumors and may serve as a tool for diagnosis and prediction. However, the status of miRNAs in ES has not yet been thoroughly investigated. This study compared global miRNAs expression in paraffin-embedded tumor tissue samples from 20 ES patients, affected by primary untreated tumors, with miRNAs expressed in normal human mesenchymal stromal cells (MSCs) by microarray analysis. A miRTarBase database was used to identify the predicted target genes for differentially expressed miRNAs. The miRNAs microarray analysis revealed distinct patterns of miRNAs expression between ES samples and normal MSCs. 58 of the 954 analyzed miRNAs were significantly differentially expressed in ES samples compared to MSCs. Moreover, the qRT-PCR analysis carried out on three selected miRNAs showed that miR-181b, miR-1915 and miR-1275 were significantly aberrantly regulated, confirming the microarray results. Bio-database analysis identified BCL-2 as a bona fide target gene of the miR-21, miR-181a, miR-181b, miR-29a, miR-29b, miR-497, miR-195, miR-let-7a, miR-34a and miR-1915. Using paraffin-embedded tissues from ES patients, this study has identified several potential target miRNAs and one gene that might be considered a novel critical biomarker for ES pathogenesis.
format Online
Article
Text
id pubmed-4881482
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-48814822016-05-27 Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue Parafioriti, Antonina Bason, Caterina Armiraglio, Elisabetta Calciano, Lucia Daolio, Primo Andrea Berardocco, Martina Di Bernardo, Andrea Colosimo, Alessia Luksch, Roberto Berardi, Anna C. Int J Mol Sci Article The molecular mechanism responsible for Ewing’s Sarcoma (ES) remains largely unknown. MicroRNAs (miRNAs), a class of small non-coding RNAs able to regulate gene expression, are deregulated in tumors and may serve as a tool for diagnosis and prediction. However, the status of miRNAs in ES has not yet been thoroughly investigated. This study compared global miRNAs expression in paraffin-embedded tumor tissue samples from 20 ES patients, affected by primary untreated tumors, with miRNAs expressed in normal human mesenchymal stromal cells (MSCs) by microarray analysis. A miRTarBase database was used to identify the predicted target genes for differentially expressed miRNAs. The miRNAs microarray analysis revealed distinct patterns of miRNAs expression between ES samples and normal MSCs. 58 of the 954 analyzed miRNAs were significantly differentially expressed in ES samples compared to MSCs. Moreover, the qRT-PCR analysis carried out on three selected miRNAs showed that miR-181b, miR-1915 and miR-1275 were significantly aberrantly regulated, confirming the microarray results. Bio-database analysis identified BCL-2 as a bona fide target gene of the miR-21, miR-181a, miR-181b, miR-29a, miR-29b, miR-497, miR-195, miR-let-7a, miR-34a and miR-1915. Using paraffin-embedded tissues from ES patients, this study has identified several potential target miRNAs and one gene that might be considered a novel critical biomarker for ES pathogenesis. MDPI 2016-04-30 /pmc/articles/PMC4881482/ /pubmed/27144561 http://dx.doi.org/10.3390/ijms17050656 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parafioriti, Antonina
Bason, Caterina
Armiraglio, Elisabetta
Calciano, Lucia
Daolio, Primo Andrea
Berardocco, Martina
Di Bernardo, Andrea
Colosimo, Alessia
Luksch, Roberto
Berardi, Anna C.
Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue
title Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue
title_full Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue
title_fullStr Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue
title_full_unstemmed Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue
title_short Ewing’s Sarcoma: An Analysis of miRNA Expression Profiles and Target Genes in Paraffin-Embedded Primary Tumor Tissue
title_sort ewing’s sarcoma: an analysis of mirna expression profiles and target genes in paraffin-embedded primary tumor tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881482/
https://www.ncbi.nlm.nih.gov/pubmed/27144561
http://dx.doi.org/10.3390/ijms17050656
work_keys_str_mv AT parafioritiantonina ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT basoncaterina ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT armiraglioelisabetta ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT calcianolucia ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT daolioprimoandrea ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT berardoccomartina ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT dibernardoandrea ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT colosimoalessia ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT lukschroberto ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue
AT berardiannac ewingssarcomaananalysisofmirnaexpressionprofilesandtargetgenesinparaffinembeddedprimarytumortissue