Cargando…

Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3

It is known that aquaporin 9 (AQP9) in the prostate was strictly upregulated by androgen and may represent a novel therapeutic target for several cancers, but whether AQP9 plays a role in the regulation of androgen-independent prostate cancer still remains unclear. In the present study, AQP9 was det...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Qiwei, Zhu, Liang, Zheng, Bo, Wang, Jinliang, Song, Xishuang, Zheng, Wei, Wang, Lina, Yang, Deyong, Wang, Jianbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881560/
https://www.ncbi.nlm.nih.gov/pubmed/27187384
http://dx.doi.org/10.3390/ijms17050738
_version_ 1782433985487437824
author Chen, Qiwei
Zhu, Liang
Zheng, Bo
Wang, Jinliang
Song, Xishuang
Zheng, Wei
Wang, Lina
Yang, Deyong
Wang, Jianbo
author_facet Chen, Qiwei
Zhu, Liang
Zheng, Bo
Wang, Jinliang
Song, Xishuang
Zheng, Wei
Wang, Lina
Yang, Deyong
Wang, Jianbo
author_sort Chen, Qiwei
collection PubMed
description It is known that aquaporin 9 (AQP9) in the prostate was strictly upregulated by androgen and may represent a novel therapeutic target for several cancers, but whether AQP9 plays a role in the regulation of androgen-independent prostate cancer still remains unclear. In the present study, AQP9 was determined in prostate cancer and adjacent cancer tissues; AQP9-siRNA was applied to silencing AQP9 in androgen-independent prostate cancer cell PC3 cell line. Western blot and flow cytometry analysis were employed to detect changes in related-function of control and AQP9-siRNA groups. The results showed that AQP9 is significantly induced in cancer tissues than that in adjacent cancer tissues. Moreover, knockdown of AQP9 in PC3 androgen-independent prostate cancer cell prostate cancer cells increased inhibition rates of proliferation. In addition, knockdown of AQP9 resulted in a significant decrease in the expression of the Bcl-2 and with a notable increase in the expression of Bax and cleaved caspase 3, indicated that AQP9 knockdown promoted apoptosis in prostate cancer cells. From wound healing assay and matrigel invasion, we suggested that AQP9 expression affects the motility and invasiveness of prostate cancer cells. Moreover, In order to explore the pathway may be involved in AQP9-mediated motility and invasion of prostate cancer cells, the phosphorylation of ERK1/2 was significant suppressed in AQP9 siRNA-transfected cells compared with that in control cells, suggesting that AQP9 is involved in the activation of the ERK pathway in androgen-independent prostate cancer cells.
format Online
Article
Text
id pubmed-4881560
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-48815602016-05-27 Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3 Chen, Qiwei Zhu, Liang Zheng, Bo Wang, Jinliang Song, Xishuang Zheng, Wei Wang, Lina Yang, Deyong Wang, Jianbo Int J Mol Sci Article It is known that aquaporin 9 (AQP9) in the prostate was strictly upregulated by androgen and may represent a novel therapeutic target for several cancers, but whether AQP9 plays a role in the regulation of androgen-independent prostate cancer still remains unclear. In the present study, AQP9 was determined in prostate cancer and adjacent cancer tissues; AQP9-siRNA was applied to silencing AQP9 in androgen-independent prostate cancer cell PC3 cell line. Western blot and flow cytometry analysis were employed to detect changes in related-function of control and AQP9-siRNA groups. The results showed that AQP9 is significantly induced in cancer tissues than that in adjacent cancer tissues. Moreover, knockdown of AQP9 in PC3 androgen-independent prostate cancer cell prostate cancer cells increased inhibition rates of proliferation. In addition, knockdown of AQP9 resulted in a significant decrease in the expression of the Bcl-2 and with a notable increase in the expression of Bax and cleaved caspase 3, indicated that AQP9 knockdown promoted apoptosis in prostate cancer cells. From wound healing assay and matrigel invasion, we suggested that AQP9 expression affects the motility and invasiveness of prostate cancer cells. Moreover, In order to explore the pathway may be involved in AQP9-mediated motility and invasion of prostate cancer cells, the phosphorylation of ERK1/2 was significant suppressed in AQP9 siRNA-transfected cells compared with that in control cells, suggesting that AQP9 is involved in the activation of the ERK pathway in androgen-independent prostate cancer cells. MDPI 2016-05-14 /pmc/articles/PMC4881560/ /pubmed/27187384 http://dx.doi.org/10.3390/ijms17050738 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Qiwei
Zhu, Liang
Zheng, Bo
Wang, Jinliang
Song, Xishuang
Zheng, Wei
Wang, Lina
Yang, Deyong
Wang, Jianbo
Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3
title Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3
title_full Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3
title_fullStr Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3
title_full_unstemmed Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3
title_short Effect of AQP9 Expression in Androgen-Independent Prostate Cancer Cell PC3
title_sort effect of aqp9 expression in androgen-independent prostate cancer cell pc3
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881560/
https://www.ncbi.nlm.nih.gov/pubmed/27187384
http://dx.doi.org/10.3390/ijms17050738
work_keys_str_mv AT chenqiwei effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT zhuliang effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT zhengbo effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT wangjinliang effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT songxishuang effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT zhengwei effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT wanglina effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT yangdeyong effectofaqp9expressioninandrogenindependentprostatecancercellpc3
AT wangjianbo effectofaqp9expressioninandrogenindependentprostatecancercellpc3