Cargando…

Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection

Nervous necrosis virus (NNV) is the causative agent of viral encephalopathy and retinopathy (VER), a neurological disease responsible for high mortality of fish species worldwide. Taking advantage of our established Chinese perch brain (CPB) cell line derived from brain tissues of Mandarin fish (Sin...

Descripción completa

Detalles Bibliográficos
Autores principales: Tu, Jiagang, Chen, Wenjie, Fu, Xiaozhe, Lin, Qiang, Chang, Ouqin, Zhao, Lijuan, Lan, Jiangfeng, Li, Ningqiu, Lin, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881562/
https://www.ncbi.nlm.nih.gov/pubmed/27213348
http://dx.doi.org/10.3390/ijms17050740
_version_ 1782433985935179776
author Tu, Jiagang
Chen, Wenjie
Fu, Xiaozhe
Lin, Qiang
Chang, Ouqin
Zhao, Lijuan
Lan, Jiangfeng
Li, Ningqiu
Lin, Li
author_facet Tu, Jiagang
Chen, Wenjie
Fu, Xiaozhe
Lin, Qiang
Chang, Ouqin
Zhao, Lijuan
Lan, Jiangfeng
Li, Ningqiu
Lin, Li
author_sort Tu, Jiagang
collection PubMed
description Nervous necrosis virus (NNV) is the causative agent of viral encephalopathy and retinopathy (VER), a neurological disease responsible for high mortality of fish species worldwide. Taking advantage of our established Chinese perch brain (CPB) cell line derived from brain tissues of Mandarin fish (Siniperca chuatsi), the susceptibility of CPB cell to Red-Spotted Grouper nervous necrosis virus (RGNNV) was evaluated. The results showed that RGNNV replicated well in CPB cells, resulting in cellular apoptosis. Moreover, the susceptibility of Mandarin fish to RGNNV was also evaluated. Abnormal swimming was observed in RGNNV-infected Mandarin fish. In addition, the cellular vacuolation and viral particles were also observed in brain tissues of RGNNV-infected Mandarin fish by Hematoxylin-eosin staining or electronic microscopy. The established RGNNV susceptible brain cell line from freshwater fish will pave a new way for the study of the pathogenicity and replication of NNV in the future.
format Online
Article
Text
id pubmed-4881562
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-48815622016-05-27 Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection Tu, Jiagang Chen, Wenjie Fu, Xiaozhe Lin, Qiang Chang, Ouqin Zhao, Lijuan Lan, Jiangfeng Li, Ningqiu Lin, Li Int J Mol Sci Article Nervous necrosis virus (NNV) is the causative agent of viral encephalopathy and retinopathy (VER), a neurological disease responsible for high mortality of fish species worldwide. Taking advantage of our established Chinese perch brain (CPB) cell line derived from brain tissues of Mandarin fish (Siniperca chuatsi), the susceptibility of CPB cell to Red-Spotted Grouper nervous necrosis virus (RGNNV) was evaluated. The results showed that RGNNV replicated well in CPB cells, resulting in cellular apoptosis. Moreover, the susceptibility of Mandarin fish to RGNNV was also evaluated. Abnormal swimming was observed in RGNNV-infected Mandarin fish. In addition, the cellular vacuolation and viral particles were also observed in brain tissues of RGNNV-infected Mandarin fish by Hematoxylin-eosin staining or electronic microscopy. The established RGNNV susceptible brain cell line from freshwater fish will pave a new way for the study of the pathogenicity and replication of NNV in the future. MDPI 2016-05-19 /pmc/articles/PMC4881562/ /pubmed/27213348 http://dx.doi.org/10.3390/ijms17050740 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tu, Jiagang
Chen, Wenjie
Fu, Xiaozhe
Lin, Qiang
Chang, Ouqin
Zhao, Lijuan
Lan, Jiangfeng
Li, Ningqiu
Lin, Li
Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection
title Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection
title_full Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection
title_fullStr Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection
title_full_unstemmed Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection
title_short Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection
title_sort susceptibility of chinese perch brain (cpb) cell and mandarin fish to red-spotted grouper nervous necrosis virus (rgnnv) infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881562/
https://www.ncbi.nlm.nih.gov/pubmed/27213348
http://dx.doi.org/10.3390/ijms17050740
work_keys_str_mv AT tujiagang susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT chenwenjie susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT fuxiaozhe susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT linqiang susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT changouqin susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT zhaolijuan susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT lanjiangfeng susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT liningqiu susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection
AT linli susceptibilityofchineseperchbraincpbcellandmandarinfishtoredspottedgroupernervousnecrosisvirusrgnnvinfection