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Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing

The link between infection with high-risk human papillomavirus (hrHPV) and cervical cancer has been clearly demonstrated. Virological end-points showing the absence of persistent HPV infection are now accepted as a way of monitoring the impact of prophylactic vaccination programs and therapeutic vac...

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Autores principales: Vorsters, Alex, Van Keer, Severien, Biesmans, Samantha, Hens, Annick, De Coster, Ilse, Goossens, Herman, Ieven, Margareta, Van Damme, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881571/
https://www.ncbi.nlm.nih.gov/pubmed/27196899
http://dx.doi.org/10.3390/ijms17050750
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author Vorsters, Alex
Van Keer, Severien
Biesmans, Samantha
Hens, Annick
De Coster, Ilse
Goossens, Herman
Ieven, Margareta
Van Damme, Pierre
author_facet Vorsters, Alex
Van Keer, Severien
Biesmans, Samantha
Hens, Annick
De Coster, Ilse
Goossens, Herman
Ieven, Margareta
Van Damme, Pierre
author_sort Vorsters, Alex
collection PubMed
description The link between infection with high-risk human papillomavirus (hrHPV) and cervical cancer has been clearly demonstrated. Virological end-points showing the absence of persistent HPV infection are now accepted as a way of monitoring the impact of prophylactic vaccination programs and therapeutic vaccine trials. This study investigated the use of urine samples, which can be collected by self-sampling at home, instead of cervical samples for follow-up of an HPV intervention trial. Eighteen initially HPV DNA-positive women participating in an HPV therapeutic vaccine trial were monitored during a three-year follow-up period. A total of 172 urine samples and 85 cervical samples were collected. We obtained a paired urine sample for each of the 85 cervical samples by recovering urine samples from six monthly gynaecological examinations. We performed a small pilot study in which the participating women used a urine collection device at home and returned their urine sample to the laboratory by mail. All samples were analyzed using quantitative real-time HPV DNA PCR. A good association (κ value of 0.65) was found between the presence of HPV DNA in urine and a subsequent cervical sample. Comparisons of the number of HPV DNA copies in urine and paired cervical samples revealed a significant Spearman rho of 0.676. This correlation was superior in women with severe lesions. The HPV DNA results of the small pilot study based on self-collected urine samples at home are consistent with previous and subsequent urine and/or cervical results. We demonstrated that urine sampling may be a valid alternative to cervical samples for the follow-up of HPV intervention trials or programs. The potential clinical value of urine viral load monitoring should be further investigated.
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spelling pubmed-48815712016-05-27 Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing Vorsters, Alex Van Keer, Severien Biesmans, Samantha Hens, Annick De Coster, Ilse Goossens, Herman Ieven, Margareta Van Damme, Pierre Int J Mol Sci Article The link between infection with high-risk human papillomavirus (hrHPV) and cervical cancer has been clearly demonstrated. Virological end-points showing the absence of persistent HPV infection are now accepted as a way of monitoring the impact of prophylactic vaccination programs and therapeutic vaccine trials. This study investigated the use of urine samples, which can be collected by self-sampling at home, instead of cervical samples for follow-up of an HPV intervention trial. Eighteen initially HPV DNA-positive women participating in an HPV therapeutic vaccine trial were monitored during a three-year follow-up period. A total of 172 urine samples and 85 cervical samples were collected. We obtained a paired urine sample for each of the 85 cervical samples by recovering urine samples from six monthly gynaecological examinations. We performed a small pilot study in which the participating women used a urine collection device at home and returned their urine sample to the laboratory by mail. All samples were analyzed using quantitative real-time HPV DNA PCR. A good association (κ value of 0.65) was found between the presence of HPV DNA in urine and a subsequent cervical sample. Comparisons of the number of HPV DNA copies in urine and paired cervical samples revealed a significant Spearman rho of 0.676. This correlation was superior in women with severe lesions. The HPV DNA results of the small pilot study based on self-collected urine samples at home are consistent with previous and subsequent urine and/or cervical results. We demonstrated that urine sampling may be a valid alternative to cervical samples for the follow-up of HPV intervention trials or programs. The potential clinical value of urine viral load monitoring should be further investigated. MDPI 2016-05-17 /pmc/articles/PMC4881571/ /pubmed/27196899 http://dx.doi.org/10.3390/ijms17050750 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vorsters, Alex
Van Keer, Severien
Biesmans, Samantha
Hens, Annick
De Coster, Ilse
Goossens, Herman
Ieven, Margareta
Van Damme, Pierre
Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing
title Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing
title_full Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing
title_fullStr Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing
title_full_unstemmed Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing
title_short Long-Term Follow-up of HPV Infection Using Urine and Cervical Quantitative HPV DNA Testing
title_sort long-term follow-up of hpv infection using urine and cervical quantitative hpv dna testing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4881571/
https://www.ncbi.nlm.nih.gov/pubmed/27196899
http://dx.doi.org/10.3390/ijms17050750
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