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A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy

Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2...

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Autores principales: Geraci, Daniela M., Giuffrè, Mario, Bonura, Celestino, Graziano, Giorgio, Saporito, Laura, Insinga, Vincenzo, Rinaudo, Grazia, Aleo, Aurora, Vecchio, Davide, Mammina, Caterina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882316/
https://www.ncbi.nlm.nih.gov/pubmed/27303395
http://dx.doi.org/10.3389/fmicb.2016.00815
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author Geraci, Daniela M.
Giuffrè, Mario
Bonura, Celestino
Graziano, Giorgio
Saporito, Laura
Insinga, Vincenzo
Rinaudo, Grazia
Aleo, Aurora
Vecchio, Davide
Mammina, Caterina
author_facet Geraci, Daniela M.
Giuffrè, Mario
Bonura, Celestino
Graziano, Giorgio
Saporito, Laura
Insinga, Vincenzo
Rinaudo, Grazia
Aleo, Aurora
Vecchio, Davide
Mammina, Caterina
author_sort Geraci, Daniela M.
collection PubMed
description Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014–January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at 4 week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF), and multilocus sequence typing (MLST). Results: In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157, and 95 from NICUs A, C, D, and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A–F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions: Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities.
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spelling pubmed-48823162016-06-14 A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy Geraci, Daniela M. Giuffrè, Mario Bonura, Celestino Graziano, Giorgio Saporito, Laura Insinga, Vincenzo Rinaudo, Grazia Aleo, Aurora Vecchio, Davide Mammina, Caterina Front Microbiol Microbiology Objectives: We performed a 1-year prospective surveillance study on MRSA colonization within the five NICUs of the metropolitan area of Palermo, Italy. The purpose of the study was to assess epidemiology of MRSA in NICU from a network perspective. Methods: Transfer of patients between NICUs during 2014 was traced based on the annual hospital discharge records. In the period February 2014–January 2015, in the NICU B, at the University teaching hospital, nasal swabs from all infants were collected weekly, whereas in the other four NICUs (A, C, D, E) at 4 week-intervals of time. MRSA isolates were submitted to antibiotic susceptibility testing, SCCmec typing, PCR to detect lukS-PV and lukF-PV (lukS/F-PV) genes and the gene encoding the toxic shock syndrome toxin (TSST-1), multilocus variable number tandem repeat fingerprinting (MLVF), and multilocus sequence typing (MLST). Results: In the period under study, 587 nasal swabs were obtained from NICU B, whereas 218, 180, 157, and 95 from NICUs A, C, D, and E, respectively. Two groups of NICUs at high prevalence and low prevalence of MRSA colonization were recognized. Overall, 113 isolates of MRSA were identified from 102 infants. Six MLVF types (A–F) were detected, with type C being subdivided into five subtypes. Five sequence types (STs) were found with ST22-IVa being the most frequent type in all NICUs. All the MRSA molecular subtypes, except for ST1-IVa, were identified in NICU B. Conclusions: Our findings support the need to approach surveillance and infection control in NICU in a network perspective, prioritizing referral healthcare facilities. Frontiers Media S.A. 2016-05-27 /pmc/articles/PMC4882316/ /pubmed/27303395 http://dx.doi.org/10.3389/fmicb.2016.00815 Text en Copyright © 2016 Geraci, Giuffrè, Bonura, Graziano, Saporito, Insinga, Rinaudo, Aleo, Vecchio, Mammina and The NICU Surveillance Study Group. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Geraci, Daniela M.
Giuffrè, Mario
Bonura, Celestino
Graziano, Giorgio
Saporito, Laura
Insinga, Vincenzo
Rinaudo, Grazia
Aleo, Aurora
Vecchio, Davide
Mammina, Caterina
A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
title A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
title_full A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
title_fullStr A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
title_full_unstemmed A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
title_short A Snapshot on MRSA Epidemiology in a Neonatal Intensive Care Unit Network, Palermo, Italy
title_sort snapshot on mrsa epidemiology in a neonatal intensive care unit network, palermo, italy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882316/
https://www.ncbi.nlm.nih.gov/pubmed/27303395
http://dx.doi.org/10.3389/fmicb.2016.00815
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