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Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes

We show that the selective localization of cholesterol-rich domains and associated ganglioside receptors prefer to occur in the monolayer across continuous monolayer-bilayer junctions (MBJs) in supported lipid membranes. For the MBJs, glass substrates were patterned with poly(dimethylsiloxane) (PDMS...

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Autores principales: Ryu, Yong-Sang, Wittenberg, Nathan J., Suh, Jeng-Hun, Lee, Sang-Wook, Sohn, Youngjoo, Oh, Sang-Hyun, Parikh, Atul N., Lee, Sin-Doo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882513/
https://www.ncbi.nlm.nih.gov/pubmed/27230411
http://dx.doi.org/10.1038/srep26823
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author Ryu, Yong-Sang
Wittenberg, Nathan J.
Suh, Jeng-Hun
Lee, Sang-Wook
Sohn, Youngjoo
Oh, Sang-Hyun
Parikh, Atul N.
Lee, Sin-Doo
author_facet Ryu, Yong-Sang
Wittenberg, Nathan J.
Suh, Jeng-Hun
Lee, Sang-Wook
Sohn, Youngjoo
Oh, Sang-Hyun
Parikh, Atul N.
Lee, Sin-Doo
author_sort Ryu, Yong-Sang
collection PubMed
description We show that the selective localization of cholesterol-rich domains and associated ganglioside receptors prefer to occur in the monolayer across continuous monolayer-bilayer junctions (MBJs) in supported lipid membranes. For the MBJs, glass substrates were patterned with poly(dimethylsiloxane) (PDMS) oligomers by thermally-assisted contact printing, leaving behind 3 nm-thick PDMS patterns. The hydrophobicity of the transferred PDMS patterns was precisely tuned by the stamping temperature. Lipid monolayers were formed on the PDMS patterned surface while lipid bilayers were on the bare glass surface. Due to the continuity of the lipid membranes over the MBJs, essentially free diffusion of lipids was allowed between the monolayer on the PDMS surface and the upper leaflet of the bilayer on the glass substrate. The preferential localization of sphingomyelin, ganglioside GM1 and cholesterol in the monolayer region enabled to develop raft microdomains through coarsening of nanorafts. Our methodology provides a simple and effective scheme of non-disruptive manipulation of the chemical landscape associated with lipid phase separations, which leads to more sophisticated applications in biosensors and as cell culture substrates.
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spelling pubmed-48825132016-06-08 Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes Ryu, Yong-Sang Wittenberg, Nathan J. Suh, Jeng-Hun Lee, Sang-Wook Sohn, Youngjoo Oh, Sang-Hyun Parikh, Atul N. Lee, Sin-Doo Sci Rep Article We show that the selective localization of cholesterol-rich domains and associated ganglioside receptors prefer to occur in the monolayer across continuous monolayer-bilayer junctions (MBJs) in supported lipid membranes. For the MBJs, glass substrates were patterned with poly(dimethylsiloxane) (PDMS) oligomers by thermally-assisted contact printing, leaving behind 3 nm-thick PDMS patterns. The hydrophobicity of the transferred PDMS patterns was precisely tuned by the stamping temperature. Lipid monolayers were formed on the PDMS patterned surface while lipid bilayers were on the bare glass surface. Due to the continuity of the lipid membranes over the MBJs, essentially free diffusion of lipids was allowed between the monolayer on the PDMS surface and the upper leaflet of the bilayer on the glass substrate. The preferential localization of sphingomyelin, ganglioside GM1 and cholesterol in the monolayer region enabled to develop raft microdomains through coarsening of nanorafts. Our methodology provides a simple and effective scheme of non-disruptive manipulation of the chemical landscape associated with lipid phase separations, which leads to more sophisticated applications in biosensors and as cell culture substrates. Nature Publishing Group 2016-05-27 /pmc/articles/PMC4882513/ /pubmed/27230411 http://dx.doi.org/10.1038/srep26823 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ryu, Yong-Sang
Wittenberg, Nathan J.
Suh, Jeng-Hun
Lee, Sang-Wook
Sohn, Youngjoo
Oh, Sang-Hyun
Parikh, Atul N.
Lee, Sin-Doo
Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
title Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
title_full Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
title_fullStr Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
title_full_unstemmed Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
title_short Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes
title_sort continuity of monolayer-bilayer junctions for localization of lipid raft microdomains in model membranes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882513/
https://www.ncbi.nlm.nih.gov/pubmed/27230411
http://dx.doi.org/10.1038/srep26823
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