A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays

DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonan...

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Detalles Bibliográficos
Autores principales: Li, Yansheng, Du, Hongwu, Wang, Wenqian, Zhang, Peixun, Xu, Liping, Wen, Yongqiang, Zhang, Xueji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882533/
https://www.ncbi.nlm.nih.gov/pubmed/27230484
http://dx.doi.org/10.1038/srep26879
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author Li, Yansheng
Du, Hongwu
Wang, Wenqian
Zhang, Peixun
Xu, Liping
Wen, Yongqiang
Zhang, Xueji
author_facet Li, Yansheng
Du, Hongwu
Wang, Wenqian
Zhang, Peixun
Xu, Liping
Wen, Yongqiang
Zhang, Xueji
author_sort Li, Yansheng
collection PubMed
description DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonance energy transfer (FRET) cascades on a linear DNA assemblies. Seven combinations of three kinds of targets are successfully detected through the changes of fluorescence spectra because of the three-steps FRET or non-FRET continuity mechanisms. This nano-assembled FRET-based nanowire is extremely significant for the development of rapid, simple and sensitive detection system. The method used here could be extended to a general platform for multiplex detection through more-step FRET process.
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spelling pubmed-48825332016-06-08 A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays Li, Yansheng Du, Hongwu Wang, Wenqian Zhang, Peixun Xu, Liping Wen, Yongqiang Zhang, Xueji Sci Rep Article DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonance energy transfer (FRET) cascades on a linear DNA assemblies. Seven combinations of three kinds of targets are successfully detected through the changes of fluorescence spectra because of the three-steps FRET or non-FRET continuity mechanisms. This nano-assembled FRET-based nanowire is extremely significant for the development of rapid, simple and sensitive detection system. The method used here could be extended to a general platform for multiplex detection through more-step FRET process. Nature Publishing Group 2016-05-27 /pmc/articles/PMC4882533/ /pubmed/27230484 http://dx.doi.org/10.1038/srep26879 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Yansheng
Du, Hongwu
Wang, Wenqian
Zhang, Peixun
Xu, Liping
Wen, Yongqiang
Zhang, Xueji
A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
title A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
title_full A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
title_fullStr A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
title_full_unstemmed A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
title_short A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
title_sort versatile multiple target detection system based on dna nano-assembled linear fret arrays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4882533/
https://www.ncbi.nlm.nih.gov/pubmed/27230484
http://dx.doi.org/10.1038/srep26879
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