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Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius

BACKGROUND: Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz (Fabaceae) is a tree which is native to Brazil, widely known as “Jucá,” where its herbal derivatives are used in folk medicine with several therapeutic properties. The constituents, which have already been described in the fruit, are mainly h...

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Autores principales: Ferreira, Magda R. A., Fernandes, Mônica T. M., da Silva, Wliana A. V., Bezerra, Isabelle C. F., de Souza, Tatiane P., Pimentel, Maria F., Soares, Luiz A. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883093/
https://www.ncbi.nlm.nih.gov/pubmed/27279721
http://dx.doi.org/10.4103/0973-1296.182165
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author Ferreira, Magda R. A.
Fernandes, Mônica T. M.
da Silva, Wliana A. V.
Bezerra, Isabelle C. F.
de Souza, Tatiane P.
Pimentel, Maria F.
Soares, Luiz A. L.
author_facet Ferreira, Magda R. A.
Fernandes, Mônica T. M.
da Silva, Wliana A. V.
Bezerra, Isabelle C. F.
de Souza, Tatiane P.
Pimentel, Maria F.
Soares, Luiz A. L.
author_sort Ferreira, Magda R. A.
collection PubMed
description BACKGROUND: Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz (Fabaceae) is a tree which is native to Brazil, widely known as “Jucá,” where its herbal derivatives are used in folk medicine with several therapeutic properties. The constituents, which have already been described in the fruit, are mainly hydrolysable tannins (gallic acid [GA] and ellagic acid [EA]). OBJECTIVE: The aim of this study was to investigate the phenolic variability in the fruit of L. ferrea by ultraviolet/visible (UV/VIS) and chromatographic methods (high-performance liquid chromatography [HPLC]/high-performance thin layer chromatography [HPTLC]). MATERIALS AND METHODS: Several samples were collected from different regions of Brazil and the qualitative (fingerprints by HPTLC and HPLC) and quantitative analysis (UV/VIS and HPLC) of polyphenols were performed. RESULTS: The HPTLC and HPLC profiles allowed separation and identification of both major analytical markers: EA and GA. The chemical profiles were similar in a number of spots or peaks for the samples, but some differences could be observed in the intensity or area of the analytical markers for HPTLC or HPLC, respectively. Regarding the quantitative analysis, the polyphenolic content by UV/VIS ranged from 13.99 to 37.86 g% expressed as GA or from 10.75 to 29.09 g% expressed as EA. The contents of EA and GA by liquid chromatography-reversed phase (LC-RP) method ranged from 0.57 to 2.68 g% and from 0.54 to 3.23 g%, respectively. CONCLUSION: The chemical profiles obtained by HPTLC or HPLC, as well as the quantitative analysis by spectrophotometry or LC-RP method, were suitable for discrimination of each herbal sample and can be used as tools for the comparative analysis of the fruits from L. ferrea. SUMMARY: The polyphenols of fruits of Libidibia ferrea can be quantified by UV/VIS and HPLC. The HPLC method was able to detect the gallic and ellagic acids in several samples of fruits of Libidibia ferrea. The phenolic profiles of fruits from Libidibia ferrea by HPTLC and HPLC were reproductible. Abbreviations used: HPTLC: high performance thin layer chromatography, HPLC: high performance liquid chromatography, UV-Vis: spectrophotometry
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spelling pubmed-48830932016-06-08 Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius Ferreira, Magda R. A. Fernandes, Mônica T. M. da Silva, Wliana A. V. Bezerra, Isabelle C. F. de Souza, Tatiane P. Pimentel, Maria F. Soares, Luiz A. L. Pharmacogn Mag Original Article BACKGROUND: Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz (Fabaceae) is a tree which is native to Brazil, widely known as “Jucá,” where its herbal derivatives are used in folk medicine with several therapeutic properties. The constituents, which have already been described in the fruit, are mainly hydrolysable tannins (gallic acid [GA] and ellagic acid [EA]). OBJECTIVE: The aim of this study was to investigate the phenolic variability in the fruit of L. ferrea by ultraviolet/visible (UV/VIS) and chromatographic methods (high-performance liquid chromatography [HPLC]/high-performance thin layer chromatography [HPTLC]). MATERIALS AND METHODS: Several samples were collected from different regions of Brazil and the qualitative (fingerprints by HPTLC and HPLC) and quantitative analysis (UV/VIS and HPLC) of polyphenols were performed. RESULTS: The HPTLC and HPLC profiles allowed separation and identification of both major analytical markers: EA and GA. The chemical profiles were similar in a number of spots or peaks for the samples, but some differences could be observed in the intensity or area of the analytical markers for HPTLC or HPLC, respectively. Regarding the quantitative analysis, the polyphenolic content by UV/VIS ranged from 13.99 to 37.86 g% expressed as GA or from 10.75 to 29.09 g% expressed as EA. The contents of EA and GA by liquid chromatography-reversed phase (LC-RP) method ranged from 0.57 to 2.68 g% and from 0.54 to 3.23 g%, respectively. CONCLUSION: The chemical profiles obtained by HPTLC or HPLC, as well as the quantitative analysis by spectrophotometry or LC-RP method, were suitable for discrimination of each herbal sample and can be used as tools for the comparative analysis of the fruits from L. ferrea. SUMMARY: The polyphenols of fruits of Libidibia ferrea can be quantified by UV/VIS and HPLC. The HPLC method was able to detect the gallic and ellagic acids in several samples of fruits of Libidibia ferrea. The phenolic profiles of fruits from Libidibia ferrea by HPTLC and HPLC were reproductible. Abbreviations used: HPTLC: high performance thin layer chromatography, HPLC: high performance liquid chromatography, UV-Vis: spectrophotometry Medknow Publications & Media Pvt Ltd 2016-05 2016-05-11 /pmc/articles/PMC4883093/ /pubmed/27279721 http://dx.doi.org/10.4103/0973-1296.182165 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution NonCommercial ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Ferreira, Magda R. A.
Fernandes, Mônica T. M.
da Silva, Wliana A. V.
Bezerra, Isabelle C. F.
de Souza, Tatiane P.
Pimentel, Maria F.
Soares, Luiz A. L.
Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius
title Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius
title_full Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius
title_fullStr Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius
title_full_unstemmed Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius
title_short Chromatographic and Spectrophotometric Analysis of Phenolic Compounds from Fruits of Libidibia ferrea Martius
title_sort chromatographic and spectrophotometric analysis of phenolic compounds from fruits of libidibia ferrea martius
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883093/
https://www.ncbi.nlm.nih.gov/pubmed/27279721
http://dx.doi.org/10.4103/0973-1296.182165
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