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Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice
BACKGROUND: Rice contains the lowest grain Fe content among cereals. One biological limiting factor is the tolerance of rice to Fe toxicity. Reverse and forward genetic screenings were used to identify tolerance to Fe toxicity in 4,500 M(4) lines irradiated by fast neutrons (FN). FINDINGS: Fe-tolera...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883132/ https://www.ncbi.nlm.nih.gov/pubmed/26054239 http://dx.doi.org/10.1186/s12284-014-0036-z |
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author | Ruengphayak, Siriphat Ruanjaichon, Vinitchan Saensuk, Chatree Phromphan, Supaporn Tragoonrung, Somvong Kongkachuichai, Ratchanee Vanavichit, Apichart |
author_facet | Ruengphayak, Siriphat Ruanjaichon, Vinitchan Saensuk, Chatree Phromphan, Supaporn Tragoonrung, Somvong Kongkachuichai, Ratchanee Vanavichit, Apichart |
author_sort | Ruengphayak, Siriphat |
collection | PubMed |
description | BACKGROUND: Rice contains the lowest grain Fe content among cereals. One biological limiting factor is the tolerance of rice to Fe toxicity. Reverse and forward genetic screenings were used to identify tolerance to Fe toxicity in 4,500 M(4) lines irradiated by fast neutrons (FN). FINDINGS: Fe-tolerant mutants were successfully isolated. In the forward screen, we selected five highly tolerant and four highly intolerant mutants based on the response of seedlings to 300 ppm Fe. Reverse screening based on the polymorphic coding sequence of seven Fe homeostatic genes detected by denaturing high performance liquid chromatography (dHPLC) revealed MuFRO1, a mutant for OsFRO1 (LOC_Os04g36720). The MuFRO1 mutant tolerated Fe toxicity in the vegetative stage and had 21-30% more grain Fe content than its wild type. All five highly Fe-tolerant mutants have the same haplotype as the MuFRO1, confirming the important role of OsFRO1 in Fe homeostasis in rice. CONCLUSIONS: FN radiation generated extreme Fe-tolerant mutants capable of tolerating different levels of Fe toxicity in the lowland rice environment. Mutants from both reverse and forward screens suggested a role for OsFRO1 in seedling tolerance to Fe toxicity. The MuFRO1 mutant could facilitate rice production in the high-Fe soil found in Southeast Asia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-014-0036-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4883132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-48831322016-06-21 Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice Ruengphayak, Siriphat Ruanjaichon, Vinitchan Saensuk, Chatree Phromphan, Supaporn Tragoonrung, Somvong Kongkachuichai, Ratchanee Vanavichit, Apichart Rice (N Y) Short Report BACKGROUND: Rice contains the lowest grain Fe content among cereals. One biological limiting factor is the tolerance of rice to Fe toxicity. Reverse and forward genetic screenings were used to identify tolerance to Fe toxicity in 4,500 M(4) lines irradiated by fast neutrons (FN). FINDINGS: Fe-tolerant mutants were successfully isolated. In the forward screen, we selected five highly tolerant and four highly intolerant mutants based on the response of seedlings to 300 ppm Fe. Reverse screening based on the polymorphic coding sequence of seven Fe homeostatic genes detected by denaturing high performance liquid chromatography (dHPLC) revealed MuFRO1, a mutant for OsFRO1 (LOC_Os04g36720). The MuFRO1 mutant tolerated Fe toxicity in the vegetative stage and had 21-30% more grain Fe content than its wild type. All five highly Fe-tolerant mutants have the same haplotype as the MuFRO1, confirming the important role of OsFRO1 in Fe homeostasis in rice. CONCLUSIONS: FN radiation generated extreme Fe-tolerant mutants capable of tolerating different levels of Fe toxicity in the lowland rice environment. Mutants from both reverse and forward screens suggested a role for OsFRO1 in seedling tolerance to Fe toxicity. The MuFRO1 mutant could facilitate rice production in the high-Fe soil found in Southeast Asia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12284-014-0036-z) contains supplementary material, which is available to authorized users. Springer US 2015-01-20 /pmc/articles/PMC4883132/ /pubmed/26054239 http://dx.doi.org/10.1186/s12284-014-0036-z Text en © Ruengphayak et al.; licensee Springer. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Short Report Ruengphayak, Siriphat Ruanjaichon, Vinitchan Saensuk, Chatree Phromphan, Supaporn Tragoonrung, Somvong Kongkachuichai, Ratchanee Vanavichit, Apichart Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
title | Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
title_full | Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
title_fullStr | Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
title_full_unstemmed | Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
title_short | Forward screening for seedling tolerance to Fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
title_sort | forward screening for seedling tolerance to fe toxicity reveals a polymorphic mutation in ferric chelate reductase in rice |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883132/ https://www.ncbi.nlm.nih.gov/pubmed/26054239 http://dx.doi.org/10.1186/s12284-014-0036-z |
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