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Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates

The immunological synapse (IS), a dynamic and organized junction between T-cells and antigen presenting cells (APCs), is critical for initiating adaptive immunity. The actin cytoskeleton plays a major role in T-cell reorganization during IS formation, and we previously reported that transgelin-2, an...

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Autores principales: Na, Bo-Ra, Kwon, Min-Sung, Chae, Myoung-Won, Kim, Hye-Ran, Kim, Chang-Hyun, Jun, Chang-Duk, Park, Zee-Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883795/
https://www.ncbi.nlm.nih.gov/pubmed/27232882
http://dx.doi.org/10.1371/journal.pone.0156429
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author Na, Bo-Ra
Kwon, Min-Sung
Chae, Myoung-Won
Kim, Hye-Ran
Kim, Chang-Hyun
Jun, Chang-Duk
Park, Zee-Yong
author_facet Na, Bo-Ra
Kwon, Min-Sung
Chae, Myoung-Won
Kim, Hye-Ran
Kim, Chang-Hyun
Jun, Chang-Duk
Park, Zee-Yong
author_sort Na, Bo-Ra
collection PubMed
description The immunological synapse (IS), a dynamic and organized junction between T-cells and antigen presenting cells (APCs), is critical for initiating adaptive immunity. The actin cytoskeleton plays a major role in T-cell reorganization during IS formation, and we previously reported that transgelin-2, an actin-binding protein expressed in T-cells, stabilizes cortical F-actin, promoting T-cell activation in response to antigen stimulation. Transgelin-2 is also highly expressed in B-cells, although no specific function has been reported. In this study, we found that deficiency in transgelin-2 (TAGLN2(-/-)) in B-cells had little effect on B-cell development and activation, as measured by the expression of CD69, MHC class II molecules, and CD80/86. Nevertheless, in B-cells, transgelin-2 accumulated in the IS during the interaction with T-cells. These results led us to hypothesize that transgelin-2 may also be involved in IS stability in B-cells, thereby influencing T-cell function. Notably, we found that transgelin-2 deficiency in B-cells reduced T-cell activation, as determined by the release of IL-2 and interferon-γ and the expression of CD69. Furthermore, the reduced T-cell activation was correlated with reduced B-cell–T-cell conjugate formation. Collectively, these results suggest that actin stability in B-cells during IS formation is critical for the initiation of adaptive T-cell immunity.
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spelling pubmed-48837952016-06-10 Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates Na, Bo-Ra Kwon, Min-Sung Chae, Myoung-Won Kim, Hye-Ran Kim, Chang-Hyun Jun, Chang-Duk Park, Zee-Yong PLoS One Research Article The immunological synapse (IS), a dynamic and organized junction between T-cells and antigen presenting cells (APCs), is critical for initiating adaptive immunity. The actin cytoskeleton plays a major role in T-cell reorganization during IS formation, and we previously reported that transgelin-2, an actin-binding protein expressed in T-cells, stabilizes cortical F-actin, promoting T-cell activation in response to antigen stimulation. Transgelin-2 is also highly expressed in B-cells, although no specific function has been reported. In this study, we found that deficiency in transgelin-2 (TAGLN2(-/-)) in B-cells had little effect on B-cell development and activation, as measured by the expression of CD69, MHC class II molecules, and CD80/86. Nevertheless, in B-cells, transgelin-2 accumulated in the IS during the interaction with T-cells. These results led us to hypothesize that transgelin-2 may also be involved in IS stability in B-cells, thereby influencing T-cell function. Notably, we found that transgelin-2 deficiency in B-cells reduced T-cell activation, as determined by the release of IL-2 and interferon-γ and the expression of CD69. Furthermore, the reduced T-cell activation was correlated with reduced B-cell–T-cell conjugate formation. Collectively, these results suggest that actin stability in B-cells during IS formation is critical for the initiation of adaptive T-cell immunity. Public Library of Science 2016-05-27 /pmc/articles/PMC4883795/ /pubmed/27232882 http://dx.doi.org/10.1371/journal.pone.0156429 Text en © 2016 Na et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Na, Bo-Ra
Kwon, Min-Sung
Chae, Myoung-Won
Kim, Hye-Ran
Kim, Chang-Hyun
Jun, Chang-Duk
Park, Zee-Yong
Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates
title Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates
title_full Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates
title_fullStr Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates
title_full_unstemmed Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates
title_short Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates
title_sort transgelin-2 in b-cells controls t-cell activation by stabilizing t cell - b cell conjugates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883795/
https://www.ncbi.nlm.nih.gov/pubmed/27232882
http://dx.doi.org/10.1371/journal.pone.0156429
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