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The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation

Despite the broadly conserved role of microtubules in chromosome segregation, we have a limited understanding of how molecular features of tubulin proteins contribute to the underlying mechanisms. Here we investigate the negatively charged carboxy-terminal tail domains (CTTs) of α- and β-tubulins, u...

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Autores principales: Fees, Colby P., Aiken, Jayne, O’Toole, Eileen T., Giddings, Thomas H., Moore, Jeffrey K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884069/
https://www.ncbi.nlm.nih.gov/pubmed/27053662
http://dx.doi.org/10.1091/mbc.E15-05-0300
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author Fees, Colby P.
Aiken, Jayne
O’Toole, Eileen T.
Giddings, Thomas H.
Moore, Jeffrey K.
author_facet Fees, Colby P.
Aiken, Jayne
O’Toole, Eileen T.
Giddings, Thomas H.
Moore, Jeffrey K.
author_sort Fees, Colby P.
collection PubMed
description Despite the broadly conserved role of microtubules in chromosome segregation, we have a limited understanding of how molecular features of tubulin proteins contribute to the underlying mechanisms. Here we investigate the negatively charged carboxy-terminal tail domains (CTTs) of α- and β-tubulins, using a series of mutants that alter or ablate CTTs in budding yeast. We find that ablating β-CTT causes elevated rates of chromosome loss and cell cycle delay. Complementary live-cell imaging and electron tomography show that β-CTT is necessary to properly position kinetochores and organize microtubules within the assembling spindle. We identify a minimal region of negatively charged amino acids that is necessary and sufficient for proper chromosome segregation and provide evidence that this function may be conserved across species. Our results provide the first in vivo evidence of a specific role for tubulin CTTs in chromosome segregation. We propose that β-CTT promotes the ordered segregation of chromosomes by stabilizing the spindle and contributing to forces that move chromosomes toward the spindle poles.
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spelling pubmed-48840692016-08-16 The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation Fees, Colby P. Aiken, Jayne O’Toole, Eileen T. Giddings, Thomas H. Moore, Jeffrey K. Mol Biol Cell Articles Despite the broadly conserved role of microtubules in chromosome segregation, we have a limited understanding of how molecular features of tubulin proteins contribute to the underlying mechanisms. Here we investigate the negatively charged carboxy-terminal tail domains (CTTs) of α- and β-tubulins, using a series of mutants that alter or ablate CTTs in budding yeast. We find that ablating β-CTT causes elevated rates of chromosome loss and cell cycle delay. Complementary live-cell imaging and electron tomography show that β-CTT is necessary to properly position kinetochores and organize microtubules within the assembling spindle. We identify a minimal region of negatively charged amino acids that is necessary and sufficient for proper chromosome segregation and provide evidence that this function may be conserved across species. Our results provide the first in vivo evidence of a specific role for tubulin CTTs in chromosome segregation. We propose that β-CTT promotes the ordered segregation of chromosomes by stabilizing the spindle and contributing to forces that move chromosomes toward the spindle poles. The American Society for Cell Biology 2016-06-01 /pmc/articles/PMC4884069/ /pubmed/27053662 http://dx.doi.org/10.1091/mbc.E15-05-0300 Text en © 2016 Fees et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Fees, Colby P.
Aiken, Jayne
O’Toole, Eileen T.
Giddings, Thomas H.
Moore, Jeffrey K.
The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
title The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
title_full The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
title_fullStr The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
title_full_unstemmed The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
title_short The negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
title_sort negatively charged carboxy-terminal tail of β-tubulin promotes proper chromosome segregation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884069/
https://www.ncbi.nlm.nih.gov/pubmed/27053662
http://dx.doi.org/10.1091/mbc.E15-05-0300
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