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Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos

The pigment pattern of many animal species is a result of the arrangement of different types of pigment-producing chromatophores. The zebrafish has three different types of chromatophores: black melanophores, yellow xanthophores, and shimmering iridophores arranged in a characteristic pattern of gol...

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Autores principales: Nord, Hanna, Dennhag, Nils, Muck, Joscha, von Hofsten, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884075/
https://www.ncbi.nlm.nih.gov/pubmed/27053658
http://dx.doi.org/10.1091/mbc.E15-12-0821
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author Nord, Hanna
Dennhag, Nils
Muck, Joscha
von Hofsten, Jonas
author_facet Nord, Hanna
Dennhag, Nils
Muck, Joscha
von Hofsten, Jonas
author_sort Nord, Hanna
collection PubMed
description The pigment pattern of many animal species is a result of the arrangement of different types of pigment-producing chromatophores. The zebrafish has three different types of chromatophores: black melanophores, yellow xanthophores, and shimmering iridophores arranged in a characteristic pattern of golden and blue horizontal stripes. In the zebrafish embryo, chromatophores derive from the neural crest cells. Using pax7a and pax7b zebrafish mutants, we identified a previously unknown requirement for Pax7 in xanthophore lineage formation. The absence of Pax7 results in a severe reduction of xanthophore precursor cells and a complete depletion of differentiated xanthophores in embryos as well as in adult zebrafish. In contrast, the melanophore lineage is increased in pax7a/pax7b double-mutant embryos and larvae, whereas juvenile and adult pax7a/pax7b double-mutant zebrafish display a severe decrease in melanophores and a pigment pattern disorganization indicative of a xanthophore- deficient phenotype. In summary, we propose a novel role for Pax7 in the early specification of chromatophore precursor cells.
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spelling pubmed-48840752016-08-16 Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos Nord, Hanna Dennhag, Nils Muck, Joscha von Hofsten, Jonas Mol Biol Cell Articles The pigment pattern of many animal species is a result of the arrangement of different types of pigment-producing chromatophores. The zebrafish has three different types of chromatophores: black melanophores, yellow xanthophores, and shimmering iridophores arranged in a characteristic pattern of golden and blue horizontal stripes. In the zebrafish embryo, chromatophores derive from the neural crest cells. Using pax7a and pax7b zebrafish mutants, we identified a previously unknown requirement for Pax7 in xanthophore lineage formation. The absence of Pax7 results in a severe reduction of xanthophore precursor cells and a complete depletion of differentiated xanthophores in embryos as well as in adult zebrafish. In contrast, the melanophore lineage is increased in pax7a/pax7b double-mutant embryos and larvae, whereas juvenile and adult pax7a/pax7b double-mutant zebrafish display a severe decrease in melanophores and a pigment pattern disorganization indicative of a xanthophore- deficient phenotype. In summary, we propose a novel role for Pax7 in the early specification of chromatophore precursor cells. The American Society for Cell Biology 2016-06-01 /pmc/articles/PMC4884075/ /pubmed/27053658 http://dx.doi.org/10.1091/mbc.E15-12-0821 Text en © 2016 Nord et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Nord, Hanna
Dennhag, Nils
Muck, Joscha
von Hofsten, Jonas
Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
title Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
title_full Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
title_fullStr Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
title_full_unstemmed Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
title_short Pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
title_sort pax7 is required for establishment of the xanthophore lineage in zebrafish embryos
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884075/
https://www.ncbi.nlm.nih.gov/pubmed/27053658
http://dx.doi.org/10.1091/mbc.E15-12-0821
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