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Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model

BACKGROUND: Although the incidence of non-melanoma skin cancer is increasing, there are no effective practical preventive measures other than avoiding sun exposure. OBJECTIVE: To elucidate the protective effect of topical application of biologically active vitamin D(3) (calcitriol) on skin cancer de...

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Autores principales: Kim, Ji Seok, Jung, Minyoung, Yoo, Jiyeon, Choi, Eung Ho, Park, Byung Cheol, Kim, Myung Hwa, Hong, Seung Phil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Dermatological Association; The Korean Society for Investigative Dermatology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884706/
https://www.ncbi.nlm.nih.gov/pubmed/27274628
http://dx.doi.org/10.5021/ad.2016.28.3.304
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author Kim, Ji Seok
Jung, Minyoung
Yoo, Jiyeon
Choi, Eung Ho
Park, Byung Cheol
Kim, Myung Hwa
Hong, Seung Phil
author_facet Kim, Ji Seok
Jung, Minyoung
Yoo, Jiyeon
Choi, Eung Ho
Park, Byung Cheol
Kim, Myung Hwa
Hong, Seung Phil
author_sort Kim, Ji Seok
collection PubMed
description BACKGROUND: Although the incidence of non-melanoma skin cancer is increasing, there are no effective practical preventive measures other than avoiding sun exposure. OBJECTIVE: To elucidate the protective effect of topical application of biologically active vitamin D(3) (calcitriol) on skin cancer development caused by exposure to ultraviolet (UV). METHODS: Groups of hairless mice were topically treated with either calcitriol or vehicle immediately after exposure to UVB and UVA three times weekly for the initial 20 weeks, and without UV exposure in the following 6 weeks. Tumor number was counted and biopsies were done for histopathologic analysis. The changes of cyclobutane pyrimidine dimer (CPD) were evaluated 1 hour and 11 hours after short term of UV exposure and application of calcitriol. For safety evaluation, blood test and body weights were evaluated at 23rd and 25th week. RESULTS: Total tumor count and number of tumors less than 3 mm in size tended to be fewer in calcitriol group, and tumors more than 3 mm in size showed significantly lower tumor formation rate in calcitriol group. Single application of calcitriol reduced CPD at 1 hour and 11 hours after UV exposure. Histopathologic analysis showed tumors with lower grade malignancy in calcitriol group which suggested a delay in tumor progression. However, serum levels of calcium and phosphate in calcitriol group were above normal range, and weight loss was found. CONCLUSION: Topical calcitriol may suppress the formation and progression of UV-induced non-melanoma skin cancer by enhancing the repair mechanism of UV damage.
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spelling pubmed-48847062016-06-03 Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model Kim, Ji Seok Jung, Minyoung Yoo, Jiyeon Choi, Eung Ho Park, Byung Cheol Kim, Myung Hwa Hong, Seung Phil Ann Dermatol Original Article BACKGROUND: Although the incidence of non-melanoma skin cancer is increasing, there are no effective practical preventive measures other than avoiding sun exposure. OBJECTIVE: To elucidate the protective effect of topical application of biologically active vitamin D(3) (calcitriol) on skin cancer development caused by exposure to ultraviolet (UV). METHODS: Groups of hairless mice were topically treated with either calcitriol or vehicle immediately after exposure to UVB and UVA three times weekly for the initial 20 weeks, and without UV exposure in the following 6 weeks. Tumor number was counted and biopsies were done for histopathologic analysis. The changes of cyclobutane pyrimidine dimer (CPD) were evaluated 1 hour and 11 hours after short term of UV exposure and application of calcitriol. For safety evaluation, blood test and body weights were evaluated at 23rd and 25th week. RESULTS: Total tumor count and number of tumors less than 3 mm in size tended to be fewer in calcitriol group, and tumors more than 3 mm in size showed significantly lower tumor formation rate in calcitriol group. Single application of calcitriol reduced CPD at 1 hour and 11 hours after UV exposure. Histopathologic analysis showed tumors with lower grade malignancy in calcitriol group which suggested a delay in tumor progression. However, serum levels of calcium and phosphate in calcitriol group were above normal range, and weight loss was found. CONCLUSION: Topical calcitriol may suppress the formation and progression of UV-induced non-melanoma skin cancer by enhancing the repair mechanism of UV damage. Korean Dermatological Association; The Korean Society for Investigative Dermatology 2016-06 2016-05-25 /pmc/articles/PMC4884706/ /pubmed/27274628 http://dx.doi.org/10.5021/ad.2016.28.3.304 Text en Copyright © 2016 The Korean Dermatological Association and The Korean Society for Investigative Dermatology http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Ji Seok
Jung, Minyoung
Yoo, Jiyeon
Choi, Eung Ho
Park, Byung Cheol
Kim, Myung Hwa
Hong, Seung Phil
Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model
title Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model
title_full Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model
title_fullStr Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model
title_full_unstemmed Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model
title_short Protective Effect of Topical Vitamin D(3) against Photocarcinogenesis in a Murine Model
title_sort protective effect of topical vitamin d(3) against photocarcinogenesis in a murine model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884706/
https://www.ncbi.nlm.nih.gov/pubmed/27274628
http://dx.doi.org/10.5021/ad.2016.28.3.304
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