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Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test

INTRODUCTION: Differentiating between cross-reactivity and double sensitization is still a challenging issue in allergology. AIM: To differentiate cross-reactions accompanying latex allergy with the use of the ISAC test. MATERIAL AND METHODS: Thirty-nine patients reporting immediate allergic reactio...

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Autores principales: Chełmińska, Marta, Specjalski, Krzysztof, Różyło, Anna, Kołakowska, Agata, Jassem, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884780/
https://www.ncbi.nlm.nih.gov/pubmed/27279821
http://dx.doi.org/10.5114/ada.2016.59154
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author Chełmińska, Marta
Specjalski, Krzysztof
Różyło, Anna
Kołakowska, Agata
Jassem, Ewa
author_facet Chełmińska, Marta
Specjalski, Krzysztof
Różyło, Anna
Kołakowska, Agata
Jassem, Ewa
author_sort Chełmińska, Marta
collection PubMed
description INTRODUCTION: Differentiating between cross-reactivity and double sensitization is still a challenging issue in allergology. AIM: To differentiate cross-reactions accompanying latex allergy with the use of the ISAC test. MATERIAL AND METHODS: Thirty-nine patients reporting immediate allergic reactions to latex were enrolled into the study (group A). The control group was comprised of 41 patients with allergic diseases not associated with latex (group B) and 20 healthy individuals (group C). Their history was recorded and skin prick tests were performed with latex, airborne and food allergens. Specific IgE against food allergens, latex (k82) and recombined latex allergens were determined. ImmunoCAP ISAC test was performed with 103 molecules. RESULTS: Sensitization to latex was found by means of skin tests in 16 cases and sIgE against latex was revealed in 12 cases (including 10 positive in both SPT and sIgE). In the ISAC test antibodies against recombined latex allergens were found in 8 patients with rHev b 6 as the most common. All the patients positive for rHev b 1, 5, 6, 8 had allergy or asymptomatic sensitization to food allergens cross-reacting with latex. Some reactions could not have been differentiated due to the lack of allergens in the ISAC test. Others, not related to latex-fruits syndrome were explained by cross-reactivity with other profilins or PR-10 proteins. CONCLUSIONS: ImmunoCAP ISAC test could be useful in differentiating between cross-reactions and double sensitizations. However, in the case of latex its advantages are limited due to a small panel of allergens.
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spelling pubmed-48847802016-06-08 Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test Chełmińska, Marta Specjalski, Krzysztof Różyło, Anna Kołakowska, Agata Jassem, Ewa Postepy Dermatol Alergol Original Paper INTRODUCTION: Differentiating between cross-reactivity and double sensitization is still a challenging issue in allergology. AIM: To differentiate cross-reactions accompanying latex allergy with the use of the ISAC test. MATERIAL AND METHODS: Thirty-nine patients reporting immediate allergic reactions to latex were enrolled into the study (group A). The control group was comprised of 41 patients with allergic diseases not associated with latex (group B) and 20 healthy individuals (group C). Their history was recorded and skin prick tests were performed with latex, airborne and food allergens. Specific IgE against food allergens, latex (k82) and recombined latex allergens were determined. ImmunoCAP ISAC test was performed with 103 molecules. RESULTS: Sensitization to latex was found by means of skin tests in 16 cases and sIgE against latex was revealed in 12 cases (including 10 positive in both SPT and sIgE). In the ISAC test antibodies against recombined latex allergens were found in 8 patients with rHev b 6 as the most common. All the patients positive for rHev b 1, 5, 6, 8 had allergy or asymptomatic sensitization to food allergens cross-reacting with latex. Some reactions could not have been differentiated due to the lack of allergens in the ISAC test. Others, not related to latex-fruits syndrome were explained by cross-reactivity with other profilins or PR-10 proteins. CONCLUSIONS: ImmunoCAP ISAC test could be useful in differentiating between cross-reactions and double sensitizations. However, in the case of latex its advantages are limited due to a small panel of allergens. Termedia Publishing House 2016-05-16 2016-04 /pmc/articles/PMC4884780/ /pubmed/27279821 http://dx.doi.org/10.5114/ada.2016.59154 Text en Copyright © 2016 Termedia Sp. z o.o http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Original Paper
Chełmińska, Marta
Specjalski, Krzysztof
Różyło, Anna
Kołakowska, Agata
Jassem, Ewa
Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test
title Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test
title_full Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test
title_fullStr Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test
title_full_unstemmed Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test
title_short Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test
title_sort differentiating of cross-reactions in patients with latex allergy with the use of isac test
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4884780/
https://www.ncbi.nlm.nih.gov/pubmed/27279821
http://dx.doi.org/10.5114/ada.2016.59154
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