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Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication

Orellanine (OR) toxin is produced by mushrooms of the genus Cortinarius which grow in North America and in Europe. OR poisoning is characterized by severe oliguric acute renal failure, with a mortality rate of 10%–30%. Diagnosis of OR poisoning currently hinges on a history of ingestion of Cortinari...

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Autores principales: Anantharam, Poojya, Shao, Dahai, Imerman, Paula M., Burrough, Eric, Schrunk, Dwayne, Sedkhuu, Tsevelmaa, Tang, Shusheng, Rumbeiha, Wilson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885072/
https://www.ncbi.nlm.nih.gov/pubmed/27213453
http://dx.doi.org/10.3390/toxins8050158
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author Anantharam, Poojya
Shao, Dahai
Imerman, Paula M.
Burrough, Eric
Schrunk, Dwayne
Sedkhuu, Tsevelmaa
Tang, Shusheng
Rumbeiha, Wilson
author_facet Anantharam, Poojya
Shao, Dahai
Imerman, Paula M.
Burrough, Eric
Schrunk, Dwayne
Sedkhuu, Tsevelmaa
Tang, Shusheng
Rumbeiha, Wilson
author_sort Anantharam, Poojya
collection PubMed
description Orellanine (OR) toxin is produced by mushrooms of the genus Cortinarius which grow in North America and in Europe. OR poisoning is characterized by severe oliguric acute renal failure, with a mortality rate of 10%–30%. Diagnosis of OR poisoning currently hinges on a history of ingestion of Cortinarius mushrooms and histopathology of renal biopsies. A key step in the diagnostic approach is analysis of tissues for OR. Currently, tissue-based analytical methods for OR are nonspecific and lack sensitivity. The objectives of this study were: (1) to develop definitive HPLC and LC-MS/MS tissue-based analytical methods for OR; and (2) to investigate toxicological effects of OR in mice. The HPLC limit of quantitation was 10 µg/g. For fortification levels of 15 µg/g to 50 µg/g OR in kidney, the relative standard deviation was between 1.3% and 9.8%, and accuracy was within 1.5% to 7.1%. A matrix-matched calibration curve was reproduced in this range with a correlation coefficient (r) of 0.97–0.99. The limit of detection was 20 ng/g for LC-MS/MS. In OR-injected mice, kidney OR concentrations were 97 ± 51 µg/g on Day 0 and 17 ± 1 µg/g on termination Day 3. Splenic and liver injuries were novel findings in this mouse model. The new tissue-based analytical tests will improve diagnosis of OR poisoning, while the mouse model has yielded new data advancing knowledge on OR-induced pathology. The new tissue-based analytical tests will improve diagnosis of OR poisoning, while the mouse model has yielded new data advancing knowledge on OR-induced pathology.
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spelling pubmed-48850722016-05-31 Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication Anantharam, Poojya Shao, Dahai Imerman, Paula M. Burrough, Eric Schrunk, Dwayne Sedkhuu, Tsevelmaa Tang, Shusheng Rumbeiha, Wilson Toxins (Basel) Article Orellanine (OR) toxin is produced by mushrooms of the genus Cortinarius which grow in North America and in Europe. OR poisoning is characterized by severe oliguric acute renal failure, with a mortality rate of 10%–30%. Diagnosis of OR poisoning currently hinges on a history of ingestion of Cortinarius mushrooms and histopathology of renal biopsies. A key step in the diagnostic approach is analysis of tissues for OR. Currently, tissue-based analytical methods for OR are nonspecific and lack sensitivity. The objectives of this study were: (1) to develop definitive HPLC and LC-MS/MS tissue-based analytical methods for OR; and (2) to investigate toxicological effects of OR in mice. The HPLC limit of quantitation was 10 µg/g. For fortification levels of 15 µg/g to 50 µg/g OR in kidney, the relative standard deviation was between 1.3% and 9.8%, and accuracy was within 1.5% to 7.1%. A matrix-matched calibration curve was reproduced in this range with a correlation coefficient (r) of 0.97–0.99. The limit of detection was 20 ng/g for LC-MS/MS. In OR-injected mice, kidney OR concentrations were 97 ± 51 µg/g on Day 0 and 17 ± 1 µg/g on termination Day 3. Splenic and liver injuries were novel findings in this mouse model. The new tissue-based analytical tests will improve diagnosis of OR poisoning, while the mouse model has yielded new data advancing knowledge on OR-induced pathology. The new tissue-based analytical tests will improve diagnosis of OR poisoning, while the mouse model has yielded new data advancing knowledge on OR-induced pathology. MDPI 2016-05-21 /pmc/articles/PMC4885072/ /pubmed/27213453 http://dx.doi.org/10.3390/toxins8050158 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Anantharam, Poojya
Shao, Dahai
Imerman, Paula M.
Burrough, Eric
Schrunk, Dwayne
Sedkhuu, Tsevelmaa
Tang, Shusheng
Rumbeiha, Wilson
Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication
title Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication
title_full Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication
title_fullStr Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication
title_full_unstemmed Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication
title_short Improved Tissue-Based Analytical Test Methods for Orellanine, a Biomarker of Cortinarius Mushroom Intoxication
title_sort improved tissue-based analytical test methods for orellanine, a biomarker of cortinarius mushroom intoxication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885072/
https://www.ncbi.nlm.nih.gov/pubmed/27213453
http://dx.doi.org/10.3390/toxins8050158
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