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Detection of phosphorylated Akt and MAPK in cell culture assays

This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite fo...

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Detalles Bibliográficos
Autores principales:	Molgaard, Simon, Ulrichsen, Maj, Olsen, Ditte, Glerup, Simon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2016
Materias:	Biochemistry, Genetics and Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885140/ https://www.ncbi.nlm.nih.gov/pubmed/27274457 http://dx.doi.org/10.1016/j.mex.2016.04.009

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