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Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution
Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic pept...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885146/ https://www.ncbi.nlm.nih.gov/pubmed/27274532 http://dx.doi.org/10.1016/j.dib.2016.05.013 |
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author | Boggs, Irina Hine, Brad Smolenski, Grant Hettinga, Kasper Zhang, Lina Wheeler, Thomas T. |
author_facet | Boggs, Irina Hine, Brad Smolenski, Grant Hettinga, Kasper Zhang, Lina Wheeler, Thomas T. |
author_sort | Boggs, Irina |
collection | PubMed |
description | Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication “Changes in the repertoire of bovine milk proteins during mammary involution” (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011. |
format | Online Article Text |
id | pubmed-4885146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-48851462016-06-07 Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution Boggs, Irina Hine, Brad Smolenski, Grant Hettinga, Kasper Zhang, Lina Wheeler, Thomas T. Data Brief Data Article Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication “Changes in the repertoire of bovine milk proteins during mammary involution” (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011. Elsevier 2016-05-13 /pmc/articles/PMC4885146/ /pubmed/27274532 http://dx.doi.org/10.1016/j.dib.2016.05.013 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Boggs, Irina Hine, Brad Smolenski, Grant Hettinga, Kasper Zhang, Lina Wheeler, Thomas T. Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
title | Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
title_full | Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
title_fullStr | Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
title_full_unstemmed | Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
title_short | Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
title_sort | proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885146/ https://www.ncbi.nlm.nih.gov/pubmed/27274532 http://dx.doi.org/10.1016/j.dib.2016.05.013 |
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