A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and select...

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Detalles Bibliográficos
Autores principales: Rodríguez, Jéssica, Mosquera, Jesús, García-Fandiño, Rebeca, Vázquez, M. Eugenio, Mascareñas, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885664/
https://www.ncbi.nlm.nih.gov/pubmed/27252825
http://dx.doi.org/10.1039/c6sc00045b
Descripción
Sumario:We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).

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