A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and select...

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Detalles Bibliográficos
Autores principales: Rodríguez, Jéssica, Mosquera, Jesús, García-Fandiño, Rebeca, Vázquez, M. Eugenio, Mascareñas, José L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885664/
https://www.ncbi.nlm.nih.gov/pubmed/27252825
http://dx.doi.org/10.1039/c6sc00045b
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author Rodríguez, Jéssica
Mosquera, Jesús
García-Fandiño, Rebeca
Vázquez, M. Eugenio
Mascareñas, José L.
author_facet Rodríguez, Jéssica
Mosquera, Jesús
García-Fandiño, Rebeca
Vázquez, M. Eugenio
Mascareñas, José L.
author_sort Rodríguez, Jéssica
collection PubMed
description We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).
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spelling pubmed-48856642016-05-30 A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves Rodríguez, Jéssica Mosquera, Jesús García-Fandiño, Rebeca Vázquez, M. Eugenio Mascareñas, José L. Chem Sci Chemistry We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG). Royal Society of Chemistry 2016-05-01 2016-02-05 /pmc/articles/PMC4885664/ /pubmed/27252825 http://dx.doi.org/10.1039/c6sc00045b Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Rodríguez, Jéssica
Mosquera, Jesús
García-Fandiño, Rebeca
Vázquez, M. Eugenio
Mascareñas, José L.
A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
title A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
title_full A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
title_fullStr A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
title_full_unstemmed A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
title_short A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves
title_sort designed dna binding motif that recognizes extended sites and spans two adjacent major grooves
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885664/
https://www.ncbi.nlm.nih.gov/pubmed/27252825
http://dx.doi.org/10.1039/c6sc00045b
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