Cargando…

Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection

Cultivated oat (Avena sativa), an important crop in many countries, can suffer significant losses through infection by the fungus Puccinia coronata, the causal agent of crown rust disease. Understanding the molecular basis of existing partial resistance to this disease might provide targets of inter...

Descripción completa

Detalles Bibliográficos
Autores principales: Loarce, Yolanda, Navas, Elisa, Paniagua, Carlos, Fominaya, Araceli, Manjón, José L., Ferrer, Esther
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885874/
https://www.ncbi.nlm.nih.gov/pubmed/27303424
http://dx.doi.org/10.3389/fpls.2016.00731
_version_ 1782434568822849536
author Loarce, Yolanda
Navas, Elisa
Paniagua, Carlos
Fominaya, Araceli
Manjón, José L.
Ferrer, Esther
author_facet Loarce, Yolanda
Navas, Elisa
Paniagua, Carlos
Fominaya, Araceli
Manjón, José L.
Ferrer, Esther
author_sort Loarce, Yolanda
collection PubMed
description Cultivated oat (Avena sativa), an important crop in many countries, can suffer significant losses through infection by the fungus Puccinia coronata, the causal agent of crown rust disease. Understanding the molecular basis of existing partial resistance to this disease might provide targets of interest for crop improvement programs. A suppressive subtractive hybridization (SSH) library was constructed using cDNA from the partially resistant oat genotype MN841801-1 after inoculation with the pathogen. A total of 929 genes returned a BLASTx hit and were annotated under different GO terms, including 139 genes previously described as participants in mechanisms related to the defense response and signal transduction. Among these were genes involved in pathogen recognition, cell-wall modification, oxidative burst/ROS scavenging, and abscisic acid biosynthesis, as well genes related to inducible defense responses mediated by salicylic and jasmonic acid (although none of which had been previously reported involved in strong responses). These findings support the hypothesis that basal defense mechanisms are the main systems operating in oat partial resistance to P. coronata. When the expression profiles of 20 selected genes were examined at different times following inoculation with the pathogen, the partially resistant genotype was much quicker in mounting a response than a susceptible genotype. Additionally, a number of genes not previously described in oat transcriptomes were identified in this work, increasing our molecular knowledge of this crop.
format Online
Article
Text
id pubmed-4885874
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-48858742016-06-14 Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection Loarce, Yolanda Navas, Elisa Paniagua, Carlos Fominaya, Araceli Manjón, José L. Ferrer, Esther Front Plant Sci Plant Science Cultivated oat (Avena sativa), an important crop in many countries, can suffer significant losses through infection by the fungus Puccinia coronata, the causal agent of crown rust disease. Understanding the molecular basis of existing partial resistance to this disease might provide targets of interest for crop improvement programs. A suppressive subtractive hybridization (SSH) library was constructed using cDNA from the partially resistant oat genotype MN841801-1 after inoculation with the pathogen. A total of 929 genes returned a BLASTx hit and were annotated under different GO terms, including 139 genes previously described as participants in mechanisms related to the defense response and signal transduction. Among these were genes involved in pathogen recognition, cell-wall modification, oxidative burst/ROS scavenging, and abscisic acid biosynthesis, as well genes related to inducible defense responses mediated by salicylic and jasmonic acid (although none of which had been previously reported involved in strong responses). These findings support the hypothesis that basal defense mechanisms are the main systems operating in oat partial resistance to P. coronata. When the expression profiles of 20 selected genes were examined at different times following inoculation with the pathogen, the partially resistant genotype was much quicker in mounting a response than a susceptible genotype. Additionally, a number of genes not previously described in oat transcriptomes were identified in this work, increasing our molecular knowledge of this crop. Frontiers Media S.A. 2016-05-31 /pmc/articles/PMC4885874/ /pubmed/27303424 http://dx.doi.org/10.3389/fpls.2016.00731 Text en Copyright © 2016 Loarce, Navas, Paniagua, Fominaya, Manjón and Ferrer. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Loarce, Yolanda
Navas, Elisa
Paniagua, Carlos
Fominaya, Araceli
Manjón, José L.
Ferrer, Esther
Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection
title Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection
title_full Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection
title_fullStr Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection
title_full_unstemmed Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection
title_short Identification of Genes in a Partially Resistant Genotype of Avena sativa Expressed in Response to Puccinia coronata Infection
title_sort identification of genes in a partially resistant genotype of avena sativa expressed in response to puccinia coronata infection
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4885874/
https://www.ncbi.nlm.nih.gov/pubmed/27303424
http://dx.doi.org/10.3389/fpls.2016.00731
work_keys_str_mv AT loarceyolanda identificationofgenesinapartiallyresistantgenotypeofavenasativaexpressedinresponsetopucciniacoronatainfection
AT navaselisa identificationofgenesinapartiallyresistantgenotypeofavenasativaexpressedinresponsetopucciniacoronatainfection
AT paniaguacarlos identificationofgenesinapartiallyresistantgenotypeofavenasativaexpressedinresponsetopucciniacoronatainfection
AT fominayaaraceli identificationofgenesinapartiallyresistantgenotypeofavenasativaexpressedinresponsetopucciniacoronatainfection
AT manjonjosel identificationofgenesinapartiallyresistantgenotypeofavenasativaexpressedinresponsetopucciniacoronatainfection
AT ferreresther identificationofgenesinapartiallyresistantgenotypeofavenasativaexpressedinresponsetopucciniacoronatainfection