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Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates
Reconstitution of total nephron segments after resection in the adult kidney has not been achieved; however, whether the neonatal kidney can maintain the capacity for neo-nephrogenesis after resection is unknown. We performed partial resection of the kidney in neonatal rats on postnatal days 1 (P1x...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886582/ https://www.ncbi.nlm.nih.gov/pubmed/27244673 http://dx.doi.org/10.1038/srep26792 |
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author | Kirita, Yuhei Kami, Daisuke Ishida, Ryo Adachi, Takaomi Tamagaki, Keiichi Matoba, Satoaki Kusaba, Tetsuro Gojo, Satoshi |
author_facet | Kirita, Yuhei Kami, Daisuke Ishida, Ryo Adachi, Takaomi Tamagaki, Keiichi Matoba, Satoaki Kusaba, Tetsuro Gojo, Satoshi |
author_sort | Kirita, Yuhei |
collection | PubMed |
description | Reconstitution of total nephron segments after resection in the adult kidney has not been achieved; however, whether the neonatal kidney can maintain the capacity for neo-nephrogenesis after resection is unknown. We performed partial resection of the kidney in neonatal rats on postnatal days 1 (P1x kidney) and 4 (P4x kidney) and examined morphological changes and relevant factors. The P1x kidney bulged into the newly formed cortex from the wound edge, while nephrogenesis failure was prominent in the P4x kidney. Twenty-eight days post-resection, the glomerular number, cortex area, and collecting duct were preserved in the P1x kidney, whereas these parameters were markedly decreased in the P4x kidney. During normal development, Six2 expression and Six2+ nephron progenitor cells in the cap mesenchyme both rapidly disappear after birth. However, time course analysis for the P1x kidney showed that Six2 expression and Six2+ cells were well preserved in the tissue surrounding the resected area even 2 days after resection. In conclusion, our results indicate that kidneys in early neonate rats retain the capability for neo-nephrogenesis after resection; however, this ability is lost soon after birth, which may be attributed to a declining amount of Six2+ cells. |
format | Online Article Text |
id | pubmed-4886582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48865822016-06-08 Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates Kirita, Yuhei Kami, Daisuke Ishida, Ryo Adachi, Takaomi Tamagaki, Keiichi Matoba, Satoaki Kusaba, Tetsuro Gojo, Satoshi Sci Rep Article Reconstitution of total nephron segments after resection in the adult kidney has not been achieved; however, whether the neonatal kidney can maintain the capacity for neo-nephrogenesis after resection is unknown. We performed partial resection of the kidney in neonatal rats on postnatal days 1 (P1x kidney) and 4 (P4x kidney) and examined morphological changes and relevant factors. The P1x kidney bulged into the newly formed cortex from the wound edge, while nephrogenesis failure was prominent in the P4x kidney. Twenty-eight days post-resection, the glomerular number, cortex area, and collecting duct were preserved in the P1x kidney, whereas these parameters were markedly decreased in the P4x kidney. During normal development, Six2 expression and Six2+ nephron progenitor cells in the cap mesenchyme both rapidly disappear after birth. However, time course analysis for the P1x kidney showed that Six2 expression and Six2+ cells were well preserved in the tissue surrounding the resected area even 2 days after resection. In conclusion, our results indicate that kidneys in early neonate rats retain the capability for neo-nephrogenesis after resection; however, this ability is lost soon after birth, which may be attributed to a declining amount of Six2+ cells. Nature Publishing Group 2016-05-31 /pmc/articles/PMC4886582/ /pubmed/27244673 http://dx.doi.org/10.1038/srep26792 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kirita, Yuhei Kami, Daisuke Ishida, Ryo Adachi, Takaomi Tamagaki, Keiichi Matoba, Satoaki Kusaba, Tetsuro Gojo, Satoshi Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates |
title | Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates |
title_full | Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates |
title_fullStr | Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates |
title_full_unstemmed | Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates |
title_short | Preserved Nephrogenesis Following Partial Nephrectomy in Early Neonates |
title_sort | preserved nephrogenesis following partial nephrectomy in early neonates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886582/ https://www.ncbi.nlm.nih.gov/pubmed/27244673 http://dx.doi.org/10.1038/srep26792 |
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