Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation

Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of Enterococcus faecalis 28...

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Autores principales: Al Atya, Ahmed K., Belguesmia, Yanath, Chataigne, Gabrielle, Ravallec, Rozenn, Vachée, Anne, Szunerits, Sabine, Boukherroub, Rabah, Drider, Djamel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886693/
https://www.ncbi.nlm.nih.gov/pubmed/27303396
http://dx.doi.org/10.3389/fmicb.2016.00817
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author Al Atya, Ahmed K.
Belguesmia, Yanath
Chataigne, Gabrielle
Ravallec, Rozenn
Vachée, Anne
Szunerits, Sabine
Boukherroub, Rabah
Drider, Djamel
author_facet Al Atya, Ahmed K.
Belguesmia, Yanath
Chataigne, Gabrielle
Ravallec, Rozenn
Vachée, Anne
Szunerits, Sabine
Boukherroub, Rabah
Drider, Djamel
author_sort Al Atya, Ahmed K.
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of Enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. The anti-Staphylococcal activity was shown in vitro by the assessment of the minimal inhibitory concentration (MIC), followed by a checkerboard and time-kill kinetics experiments. The data unveiled a clear synergistic effect of enterocins DD28 and DD93 in combination with erythromycin or kanamycin against the clinical MRSA-S1 strain. Besides, these combinations impeded as well the MRSA-S1 clinical strain to setup biofilms on stainless steel and glace devices.
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spelling pubmed-48866932016-06-14 Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation Al Atya, Ahmed K. Belguesmia, Yanath Chataigne, Gabrielle Ravallec, Rozenn Vachée, Anne Szunerits, Sabine Boukherroub, Rabah Drider, Djamel Front Microbiol Microbiology Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of Enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. The anti-Staphylococcal activity was shown in vitro by the assessment of the minimal inhibitory concentration (MIC), followed by a checkerboard and time-kill kinetics experiments. The data unveiled a clear synergistic effect of enterocins DD28 and DD93 in combination with erythromycin or kanamycin against the clinical MRSA-S1 strain. Besides, these combinations impeded as well the MRSA-S1 clinical strain to setup biofilms on stainless steel and glace devices. Frontiers Media S.A. 2016-05-31 /pmc/articles/PMC4886693/ /pubmed/27303396 http://dx.doi.org/10.3389/fmicb.2016.00817 Text en Copyright © 2016 Al Atya, Belguesmia, Chataigne, Ravallec, Vachée, Szunerits, Boukherroub and Drider. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Al Atya, Ahmed K.
Belguesmia, Yanath
Chataigne, Gabrielle
Ravallec, Rozenn
Vachée, Anne
Szunerits, Sabine
Boukherroub, Rabah
Drider, Djamel
Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
title Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
title_full Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
title_fullStr Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
title_full_unstemmed Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
title_short Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation
title_sort anti-mrsa activities of enterocins dd28 and dd93 and evidences on their role in the inhibition of biofilm formation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886693/
https://www.ncbi.nlm.nih.gov/pubmed/27303396
http://dx.doi.org/10.3389/fmicb.2016.00817
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