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Modeling truncated pixel values of faint reflections in MicroED images
The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886988/ https://www.ncbi.nlm.nih.gov/pubmed/27275145 http://dx.doi.org/10.1107/S1600576716007196 |
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author | Hattne, Johan Shi, Dan de la Cruz, M. Jason Reyes, Francis E. Gonen, Tamir |
author_facet | Hattne, Johan Shi, Dan de la Cruz, M. Jason Reyes, Francis E. Gonen, Tamir |
author_sort | Hattne, Johan |
collection | PubMed |
description | The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images. |
format | Online Article Text |
id | pubmed-4886988 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-48869882016-06-06 Modeling truncated pixel values of faint reflections in MicroED images Hattne, Johan Shi, Dan de la Cruz, M. Jason Reyes, Francis E. Gonen, Tamir J Appl Crystallogr Research Papers The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images. International Union of Crystallography 2016-05-11 /pmc/articles/PMC4886988/ /pubmed/27275145 http://dx.doi.org/10.1107/S1600576716007196 Text en © Johan Hattne et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Hattne, Johan Shi, Dan de la Cruz, M. Jason Reyes, Francis E. Gonen, Tamir Modeling truncated pixel values of faint reflections in MicroED images |
title | Modeling truncated pixel values of faint reflections in MicroED images
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title_full | Modeling truncated pixel values of faint reflections in MicroED images
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title_fullStr | Modeling truncated pixel values of faint reflections in MicroED images
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title_full_unstemmed | Modeling truncated pixel values of faint reflections in MicroED images
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title_short | Modeling truncated pixel values of faint reflections in MicroED images
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title_sort | modeling truncated pixel values of faint reflections in microed images |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886988/ https://www.ncbi.nlm.nih.gov/pubmed/27275145 http://dx.doi.org/10.1107/S1600576716007196 |
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