Cargando…

Modeling truncated pixel values of faint reflections in MicroED images

The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity...

Descripción completa

Detalles Bibliográficos
Autores principales: Hattne, Johan, Shi, Dan, de la Cruz, M. Jason, Reyes, Francis E., Gonen, Tamir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886988/
https://www.ncbi.nlm.nih.gov/pubmed/27275145
http://dx.doi.org/10.1107/S1600576716007196
_version_ 1782434676371095552
author Hattne, Johan
Shi, Dan
de la Cruz, M. Jason
Reyes, Francis E.
Gonen, Tamir
author_facet Hattne, Johan
Shi, Dan
de la Cruz, M. Jason
Reyes, Francis E.
Gonen, Tamir
author_sort Hattne, Johan
collection PubMed
description The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images.
format Online
Article
Text
id pubmed-4886988
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher International Union of Crystallography
record_format MEDLINE/PubMed
spelling pubmed-48869882016-06-06 Modeling truncated pixel values of faint reflections in MicroED images Hattne, Johan Shi, Dan de la Cruz, M. Jason Reyes, Francis E. Gonen, Tamir J Appl Crystallogr Research Papers The weak pixel counts surrounding the Bragg spots in a diffraction image are important for establishing a model of the background underneath the peak and estimating the reliability of the integrated intensities. Under certain circumstances, particularly with equipment not optimized for low-intensity measurements, these pixel values may be corrupted by corrections applied to the raw image. This can lead to truncation of low pixel counts, resulting in anomalies in the integrated Bragg intensities, such as systematically higher signal-to-noise ratios. A correction for this effect can be approximated by a three-parameter lognormal distribution fitted to the weakly positive-valued pixels at similar scattering angles. The procedure is validated by the improved refinement of an atomic model against structure factor amplitudes derived from corrected micro-electron diffraction (MicroED) images. International Union of Crystallography 2016-05-11 /pmc/articles/PMC4886988/ /pubmed/27275145 http://dx.doi.org/10.1107/S1600576716007196 Text en © Johan Hattne et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Hattne, Johan
Shi, Dan
de la Cruz, M. Jason
Reyes, Francis E.
Gonen, Tamir
Modeling truncated pixel values of faint reflections in MicroED images
title Modeling truncated pixel values of faint reflections in MicroED images
title_full Modeling truncated pixel values of faint reflections in MicroED images
title_fullStr Modeling truncated pixel values of faint reflections in MicroED images
title_full_unstemmed Modeling truncated pixel values of faint reflections in MicroED images
title_short Modeling truncated pixel values of faint reflections in MicroED images
title_sort modeling truncated pixel values of faint reflections in microed images
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4886988/
https://www.ncbi.nlm.nih.gov/pubmed/27275145
http://dx.doi.org/10.1107/S1600576716007196
work_keys_str_mv AT hattnejohan modelingtruncatedpixelvaluesoffaintreflectionsinmicroedimages
AT shidan modelingtruncatedpixelvaluesoffaintreflectionsinmicroedimages
AT delacruzmjason modelingtruncatedpixelvaluesoffaintreflectionsinmicroedimages
AT reyesfrancise modelingtruncatedpixelvaluesoffaintreflectionsinmicroedimages
AT gonentamir modelingtruncatedpixelvaluesoffaintreflectionsinmicroedimages