Data on the identity and myristoylation state of recombinant, purified hippocalcin

In this data article we report on the purity and post translation modification of bacterially expressed and purified recombinant hippocalcin (HPCA): a member of the neuronal calcium sensor protein family, whose functions are regulated by calcium. MALDI-TOF in source decay (ISD) analysis was used to...

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Detalles Bibliográficos
Autores principales: Krishnan, Anuradha, Viviano, Jeffrey, Morozov, Yaroslav, Venkataraman, Venkat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Proteomics and Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887589/
https://www.ncbi.nlm.nih.gov/pubmed/27284566
http://dx.doi.org/10.1016/j.dib.2016.04.024
Descripción
Sumario:In this data article we report on the purity and post translation modification of bacterially expressed and purified recombinant hippocalcin (HPCA): a member of the neuronal calcium sensor protein family, whose functions are regulated by calcium. MALDI-TOF in source decay (ISD) analysis was used to identify both the myristoylated or non-myristoylated forms of the protein. MALDI-TOF ISD data on the identity of the protein, amino acid sequence and myristoylation efficiency are provided. This data relates to the article “Single-Column Purification of the Tag-free, Recombinant Form of the Neuronal Calcium Sensor Protein, Hippocalcin Expressed in Eschericia coli” [1].

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