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Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX

Despite the advancement of clinical and preclinical research on PCa, which resulted in the last five years in a decrement of disease incidence by 3-4%, it remains the most frequent cancer in men and the second for mortality rate. Based on this evidence we present a brief dissertation on numerous pre...

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Autores principales: Rea, Domenica, del Vecchio, Vitale, Palma, Giuseppe, Barbieri, Antonio, Falco, Michela, Luciano, Antonio, De Biase, Davide, Perdonà, Sisto, Facchini, Gaetano, Arra, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887629/
https://www.ncbi.nlm.nih.gov/pubmed/27294148
http://dx.doi.org/10.1155/2016/9750795
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author Rea, Domenica
del Vecchio, Vitale
Palma, Giuseppe
Barbieri, Antonio
Falco, Michela
Luciano, Antonio
De Biase, Davide
Perdonà, Sisto
Facchini, Gaetano
Arra, Claudio
author_facet Rea, Domenica
del Vecchio, Vitale
Palma, Giuseppe
Barbieri, Antonio
Falco, Michela
Luciano, Antonio
De Biase, Davide
Perdonà, Sisto
Facchini, Gaetano
Arra, Claudio
author_sort Rea, Domenica
collection PubMed
description Despite the advancement of clinical and preclinical research on PCa, which resulted in the last five years in a decrement of disease incidence by 3-4%, it remains the most frequent cancer in men and the second for mortality rate. Based on this evidence we present a brief dissertation on numerous preclinical models, comparing their advantages and disadvantages; among this we report the PDX mouse models that show greater fidelity to the disease, in terms of histopathologic features of implanted tumor, gene and miRNA expression, and metastatic pattern, well describing all tumor progression stages; this characteristic encourages the translation of preclinical results. These models become particularly useful in meeting the need of new treatments identification that eradicate PCa bone metastases growing, clarifying pathway of angiogenesis, identifying castration-resistant stem-like cells, and studying the antiandrogen therapies. Also of considerable interest are the studies of 3D cell cultures derived from PDX, which have the ability to maintain PDX cell viability with continued native androgen receptor expression, also showing a differential sensitivity to drugs. 3D PDX PCa may represent a diagnostic platform for the rapid assessment of drugs and push personalized medicine. Today the development of preclinical models in vitro and in vivo is necessary in order to obtain increasingly reliable answers before reaching phase III of the drug discovery.
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spelling pubmed-48876292016-06-12 Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX Rea, Domenica del Vecchio, Vitale Palma, Giuseppe Barbieri, Antonio Falco, Michela Luciano, Antonio De Biase, Davide Perdonà, Sisto Facchini, Gaetano Arra, Claudio Biomed Res Int Review Article Despite the advancement of clinical and preclinical research on PCa, which resulted in the last five years in a decrement of disease incidence by 3-4%, it remains the most frequent cancer in men and the second for mortality rate. Based on this evidence we present a brief dissertation on numerous preclinical models, comparing their advantages and disadvantages; among this we report the PDX mouse models that show greater fidelity to the disease, in terms of histopathologic features of implanted tumor, gene and miRNA expression, and metastatic pattern, well describing all tumor progression stages; this characteristic encourages the translation of preclinical results. These models become particularly useful in meeting the need of new treatments identification that eradicate PCa bone metastases growing, clarifying pathway of angiogenesis, identifying castration-resistant stem-like cells, and studying the antiandrogen therapies. Also of considerable interest are the studies of 3D cell cultures derived from PDX, which have the ability to maintain PDX cell viability with continued native androgen receptor expression, also showing a differential sensitivity to drugs. 3D PDX PCa may represent a diagnostic platform for the rapid assessment of drugs and push personalized medicine. Today the development of preclinical models in vitro and in vivo is necessary in order to obtain increasingly reliable answers before reaching phase III of the drug discovery. Hindawi Publishing Corporation 2016 2016-05-18 /pmc/articles/PMC4887629/ /pubmed/27294148 http://dx.doi.org/10.1155/2016/9750795 Text en Copyright © 2016 Domenica Rea et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Rea, Domenica
del Vecchio, Vitale
Palma, Giuseppe
Barbieri, Antonio
Falco, Michela
Luciano, Antonio
De Biase, Davide
Perdonà, Sisto
Facchini, Gaetano
Arra, Claudio
Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
title Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
title_full Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
title_fullStr Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
title_full_unstemmed Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
title_short Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
title_sort mouse models in prostate cancer translational research: from xenograft to pdx
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4887629/
https://www.ncbi.nlm.nih.gov/pubmed/27294148
http://dx.doi.org/10.1155/2016/9750795
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