Cargando…
Effects of Linderae radix extracts on a rat model of alcoholic liver injury
Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of Lindera aggregata (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury....
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888052/ https://www.ncbi.nlm.nih.gov/pubmed/27313665 http://dx.doi.org/10.3892/etm.2016.3244 |
_version_ | 1782434813880303616 |
---|---|
author | WANG, JUN-WEI CHEN, XIN-YI HU, PEI-YANG TAN, MING-MING TANG, XIAO-GANG HUANG, MIN-CONG LOU, ZHAO-HUAN |
author_facet | WANG, JUN-WEI CHEN, XIN-YI HU, PEI-YANG TAN, MING-MING TANG, XIAO-GANG HUANG, MIN-CONG LOU, ZHAO-HUAN |
author_sort | WANG, JUN-WEI |
collection | PubMed |
description | Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of Lindera aggregata (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol-induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin-eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription-quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF-κB, TNF-α and IL-1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action. |
format | Online Article Text |
id | pubmed-4888052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-48880522016-06-16 Effects of Linderae radix extracts on a rat model of alcoholic liver injury WANG, JUN-WEI CHEN, XIN-YI HU, PEI-YANG TAN, MING-MING TANG, XIAO-GANG HUANG, MIN-CONG LOU, ZHAO-HUAN Exp Ther Med Articles Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root of Lindera aggregata (Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol-induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin-eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription-quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF-κB, TNF-α and IL-1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action. D.A. Spandidos 2016-06 2016-04-11 /pmc/articles/PMC4888052/ /pubmed/27313665 http://dx.doi.org/10.3892/etm.2016.3244 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles WANG, JUN-WEI CHEN, XIN-YI HU, PEI-YANG TAN, MING-MING TANG, XIAO-GANG HUANG, MIN-CONG LOU, ZHAO-HUAN Effects of Linderae radix extracts on a rat model of alcoholic liver injury |
title | Effects of Linderae radix extracts on a rat model of alcoholic liver injury |
title_full | Effects of Linderae radix extracts on a rat model of alcoholic liver injury |
title_fullStr | Effects of Linderae radix extracts on a rat model of alcoholic liver injury |
title_full_unstemmed | Effects of Linderae radix extracts on a rat model of alcoholic liver injury |
title_short | Effects of Linderae radix extracts on a rat model of alcoholic liver injury |
title_sort | effects of linderae radix extracts on a rat model of alcoholic liver injury |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888052/ https://www.ncbi.nlm.nih.gov/pubmed/27313665 http://dx.doi.org/10.3892/etm.2016.3244 |
work_keys_str_mv | AT wangjunwei effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury AT chenxinyi effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury AT hupeiyang effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury AT tanmingming effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury AT tangxiaogang effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury AT huangmincong effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury AT louzhaohuan effectsoflinderaeradixextractsonaratmodelofalcoholicliverinjury |