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Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey
BACKGROUND: In this study, our aim was to identify Candida species isolated from bloodstream infections and to determine their susceptibilities to various antifungal agents to demonstrate the local resistance profiles and to guide empirical treatment for clinicians. METHODS: Two hundred Candida isol...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888423/ https://www.ncbi.nlm.nih.gov/pubmed/27245756 http://dx.doi.org/10.1186/s12941-016-0153-1 |
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author | Dagi, Hatice Turk Findik, Duygu Senkeles, Cigdem Arslan, Ugur |
author_facet | Dagi, Hatice Turk Findik, Duygu Senkeles, Cigdem Arslan, Ugur |
author_sort | Dagi, Hatice Turk |
collection | PubMed |
description | BACKGROUND: In this study, our aim was to identify Candida species isolated from bloodstream infections and to determine their susceptibilities to various antifungal agents to demonstrate the local resistance profiles and to guide empirical treatment for clinicians. METHODS: Two hundred Candida isolates (95 Candida albicans, 105 non-albicans Candida strains) were included in the study. Candida species were identified by conventional, biochemical and molecular methods. Antifungal susceptibility tests for amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin and anidulafungin were performed with broth microdilution method according to the Clinical and Laboratory Standards Institute M27-A3 document. RESULTS: Of the 200 Candida strains, the most prevalent species were C. albicans (47.5 %), Candida glabrata (18.0 %) and Candida parapsilosis complex (14.0 %). All Candida species except for three (1.5 %) Candida kefyr strains were susceptible to amphotericin B. Only one (2.8 %) C. glabrata was resistant to fluconazole (MIC ≥ 64 μg/ml), and the others (97.2 %) exhibited dose-dependent susceptibility. All species, but C. glabrata strains, were susceptible to fluconazole. Resistance to voriconazole, posaconazole, caspofungin and anidulafungin was not detected in any strain. CONCLUSION: Candida albicans were susceptible to all antifungal drugs. Three C. kefyr strains were resistant to amphotericin B. Only one C. glabrata was resistant to fluconazole. All the strains were susceptible to voriconazole, posaconazole, caspofungin and anidulafungin. In vitro antifungal susceptibility tests should be performed to select of appropriate and effective antifungal therapy, and monitor the development of resistance. |
format | Online Article Text |
id | pubmed-4888423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48884232016-06-02 Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey Dagi, Hatice Turk Findik, Duygu Senkeles, Cigdem Arslan, Ugur Ann Clin Microbiol Antimicrob Research BACKGROUND: In this study, our aim was to identify Candida species isolated from bloodstream infections and to determine their susceptibilities to various antifungal agents to demonstrate the local resistance profiles and to guide empirical treatment for clinicians. METHODS: Two hundred Candida isolates (95 Candida albicans, 105 non-albicans Candida strains) were included in the study. Candida species were identified by conventional, biochemical and molecular methods. Antifungal susceptibility tests for amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin and anidulafungin were performed with broth microdilution method according to the Clinical and Laboratory Standards Institute M27-A3 document. RESULTS: Of the 200 Candida strains, the most prevalent species were C. albicans (47.5 %), Candida glabrata (18.0 %) and Candida parapsilosis complex (14.0 %). All Candida species except for three (1.5 %) Candida kefyr strains were susceptible to amphotericin B. Only one (2.8 %) C. glabrata was resistant to fluconazole (MIC ≥ 64 μg/ml), and the others (97.2 %) exhibited dose-dependent susceptibility. All species, but C. glabrata strains, were susceptible to fluconazole. Resistance to voriconazole, posaconazole, caspofungin and anidulafungin was not detected in any strain. CONCLUSION: Candida albicans were susceptible to all antifungal drugs. Three C. kefyr strains were resistant to amphotericin B. Only one C. glabrata was resistant to fluconazole. All the strains were susceptible to voriconazole, posaconazole, caspofungin and anidulafungin. In vitro antifungal susceptibility tests should be performed to select of appropriate and effective antifungal therapy, and monitor the development of resistance. BioMed Central 2016-05-31 /pmc/articles/PMC4888423/ /pubmed/27245756 http://dx.doi.org/10.1186/s12941-016-0153-1 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Dagi, Hatice Turk Findik, Duygu Senkeles, Cigdem Arslan, Ugur Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey |
title | Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey |
title_full | Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey |
title_fullStr | Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey |
title_full_unstemmed | Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey |
title_short | Identification and antifungal susceptibility of Candida species isolated from bloodstream infections in Konya, Turkey |
title_sort | identification and antifungal susceptibility of candida species isolated from bloodstream infections in konya, turkey |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888423/ https://www.ncbi.nlm.nih.gov/pubmed/27245756 http://dx.doi.org/10.1186/s12941-016-0153-1 |
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