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Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells

The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficult-to-transfect cells. It is well known that human, mouse, and rat skelet...

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Autores principales: Pereyra, Andrea Soledad, Mykhaylyk, Olga, Lockhart, Eugenia Falomir, Taylor, Jackson Richard, Delbono, Osvaldo, Goya, Rodolfo Gustavo, Plank, Christian, Hereñu, Claudia Beatriz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888903/
https://www.ncbi.nlm.nih.gov/pubmed/27274908
http://dx.doi.org/10.4172/2157-7439.1000364
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author Pereyra, Andrea Soledad
Mykhaylyk, Olga
Lockhart, Eugenia Falomir
Taylor, Jackson Richard
Delbono, Osvaldo
Goya, Rodolfo Gustavo
Plank, Christian
Hereñu, Claudia Beatriz
author_facet Pereyra, Andrea Soledad
Mykhaylyk, Olga
Lockhart, Eugenia Falomir
Taylor, Jackson Richard
Delbono, Osvaldo
Goya, Rodolfo Gustavo
Plank, Christian
Hereñu, Claudia Beatriz
author_sort Pereyra, Andrea Soledad
collection PubMed
description The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficult-to-transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction resistance in skeletal muscle cells. Expression of Green Fluorescent Protein and Insulin-like Growth Factor 1 was significantly enhanced after magnetofection with RAd-MNPs complexes in C2C12 myotubes in vitro and mouse skeletal muscle in vivo when compared to transduction with naked virus. These results provide evidence that magnetofection, mainly due to its membrane-receptor independent mechanism, constitutes a simple and effective alternative to current methods for gene transfer into traditionally hard-to-transfect biological models.
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spelling pubmed-48889032016-06-01 Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells Pereyra, Andrea Soledad Mykhaylyk, Olga Lockhart, Eugenia Falomir Taylor, Jackson Richard Delbono, Osvaldo Goya, Rodolfo Gustavo Plank, Christian Hereñu, Claudia Beatriz J Nanomed Nanotechnol Article The goal of magnetic field-assisted gene transfer is to enhance internalization of exogenous nucleic acids by association with magnetic nanoparticles (MNPs). This technique named magnetofection is particularly useful in difficult-to-transfect cells. It is well known that human, mouse, and rat skeletal muscle cells suffer a maturation-dependent loss of susceptibility to Recombinant Adenoviral vector (RAd) uptake. In postnatal, fully differentiated myofibers, the expression of the primary Coxsackie and Adenoviral membrane receptor (CAR) is severely downregulated representing a main hurdle for the use of these vectors in gene transfer/therapy. Here we demonstrate that assembling of Recombinant Adenoviral vectors with suitable iron oxide MNPs into magneto-adenovectors (RAd-MNP) and further exposure to a gradient magnetic field enables to efficiently overcome transduction resistance in skeletal muscle cells. Expression of Green Fluorescent Protein and Insulin-like Growth Factor 1 was significantly enhanced after magnetofection with RAd-MNPs complexes in C2C12 myotubes in vitro and mouse skeletal muscle in vivo when compared to transduction with naked virus. These results provide evidence that magnetofection, mainly due to its membrane-receptor independent mechanism, constitutes a simple and effective alternative to current methods for gene transfer into traditionally hard-to-transfect biological models. 2016-04-05 2016-04 /pmc/articles/PMC4888903/ /pubmed/27274908 http://dx.doi.org/10.4172/2157-7439.1000364 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Pereyra, Andrea Soledad
Mykhaylyk, Olga
Lockhart, Eugenia Falomir
Taylor, Jackson Richard
Delbono, Osvaldo
Goya, Rodolfo Gustavo
Plank, Christian
Hereñu, Claudia Beatriz
Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
title Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
title_full Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
title_fullStr Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
title_full_unstemmed Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
title_short Magnetofection Enhances Adenoviral Vector-based Gene Delivery in Skeletal Muscle Cells
title_sort magnetofection enhances adenoviral vector-based gene delivery in skeletal muscle cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4888903/
https://www.ncbi.nlm.nih.gov/pubmed/27274908
http://dx.doi.org/10.4172/2157-7439.1000364
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