Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions

Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL...

Descripción completa

Detalles Bibliográficos
Autores principales: Skvortsova, Yulia V., Kondratieva, Sofia A., Zinovyeva, Marina V., Nikolaev, Lev G., Azhikina, Tatyana L., Gainetdinov, Ildar V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889060/
https://www.ncbi.nlm.nih.gov/pubmed/27248499
http://dx.doi.org/10.1371/journal.pone.0156454
Descripción
Sumario:Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression.

Ejemplares similares