Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions

Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL...

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Autores principales: Skvortsova, Yulia V., Kondratieva, Sofia A., Zinovyeva, Marina V., Nikolaev, Lev G., Azhikina, Tatyana L., Gainetdinov, Ildar V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889060/
https://www.ncbi.nlm.nih.gov/pubmed/27248499
http://dx.doi.org/10.1371/journal.pone.0156454
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author Skvortsova, Yulia V.
Kondratieva, Sofia A.
Zinovyeva, Marina V.
Nikolaev, Lev G.
Azhikina, Tatyana L.
Gainetdinov, Ildar V.
author_facet Skvortsova, Yulia V.
Kondratieva, Sofia A.
Zinovyeva, Marina V.
Nikolaev, Lev G.
Azhikina, Tatyana L.
Gainetdinov, Ildar V.
author_sort Skvortsova, Yulia V.
collection PubMed
description Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression.
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spelling pubmed-48890602016-06-10 Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions Skvortsova, Yulia V. Kondratieva, Sofia A. Zinovyeva, Marina V. Nikolaev, Lev G. Azhikina, Tatyana L. Gainetdinov, Ildar V. PLoS One Research Article Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression. Public Library of Science 2016-06-01 /pmc/articles/PMC4889060/ /pubmed/27248499 http://dx.doi.org/10.1371/journal.pone.0156454 Text en © 2016 Skvortsova et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Skvortsova, Yulia V.
Kondratieva, Sofia A.
Zinovyeva, Marina V.
Nikolaev, Lev G.
Azhikina, Tatyana L.
Gainetdinov, Ildar V.
Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions
title Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions
title_full Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions
title_fullStr Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions
title_full_unstemmed Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions
title_short Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions
title_sort intragenic locus in human piwil2 gene shares promoter and enhancer functions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889060/
https://www.ncbi.nlm.nih.gov/pubmed/27248499
http://dx.doi.org/10.1371/journal.pone.0156454
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