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Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing

Melioidosis is an infectious disease caused by the saprophytic bacterium Burkholderia pseudomallei. In northeast Thailand and northern Australia, where the disease is highly endemic, a range of molecular tools have been used to study its epidemiology and pathogenesis. In the Lao People's Democr...

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Autores principales: Rachlin, Audrey, Dittrich, Sabine, Phommasone, Koukeo, Douangnouvong, Anousone, Phetsouvanh, Rattanaphone, Newton, Paul N., Dance, David A. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889735/
https://www.ncbi.nlm.nih.gov/pubmed/27001759
http://dx.doi.org/10.4269/ajtmh.15-0909
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author Rachlin, Audrey
Dittrich, Sabine
Phommasone, Koukeo
Douangnouvong, Anousone
Phetsouvanh, Rattanaphone
Newton, Paul N.
Dance, David A. B.
author_facet Rachlin, Audrey
Dittrich, Sabine
Phommasone, Koukeo
Douangnouvong, Anousone
Phetsouvanh, Rattanaphone
Newton, Paul N.
Dance, David A. B.
author_sort Rachlin, Audrey
collection PubMed
description Melioidosis is an infectious disease caused by the saprophytic bacterium Burkholderia pseudomallei. In northeast Thailand and northern Australia, where the disease is highly endemic, a range of molecular tools have been used to study its epidemiology and pathogenesis. In the Lao People's Democratic Republic (Laos) where melioidosis has been recognized as endemic since 1999, no such studies have been undertaken. We used a multilocus sequence typing scheme specific for B. pseudomallei to investigate nine cases of culture-positive recurrence occurring in 514 patients with melioidosis between 2010 and 2015: four were suspected to be relapses while the other five represented reinfections. In addition, two novel sequence types of the bacterium were identified. The low overall recurrence rates (2.4%) and proportions of relapse and reinfection in the Laos are consistent with those described in the recent literature, reflecting the effective use of appropriate antimicrobial therapy.
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spelling pubmed-48897352016-06-16 Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing Rachlin, Audrey Dittrich, Sabine Phommasone, Koukeo Douangnouvong, Anousone Phetsouvanh, Rattanaphone Newton, Paul N. Dance, David A. B. Am J Trop Med Hyg Articles Melioidosis is an infectious disease caused by the saprophytic bacterium Burkholderia pseudomallei. In northeast Thailand and northern Australia, where the disease is highly endemic, a range of molecular tools have been used to study its epidemiology and pathogenesis. In the Lao People's Democratic Republic (Laos) where melioidosis has been recognized as endemic since 1999, no such studies have been undertaken. We used a multilocus sequence typing scheme specific for B. pseudomallei to investigate nine cases of culture-positive recurrence occurring in 514 patients with melioidosis between 2010 and 2015: four were suspected to be relapses while the other five represented reinfections. In addition, two novel sequence types of the bacterium were identified. The low overall recurrence rates (2.4%) and proportions of relapse and reinfection in the Laos are consistent with those described in the recent literature, reflecting the effective use of appropriate antimicrobial therapy. The American Society of Tropical Medicine and Hygiene 2016-06-01 /pmc/articles/PMC4889735/ /pubmed/27001759 http://dx.doi.org/10.4269/ajtmh.15-0909 Text en ©The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
Rachlin, Audrey
Dittrich, Sabine
Phommasone, Koukeo
Douangnouvong, Anousone
Phetsouvanh, Rattanaphone
Newton, Paul N.
Dance, David A. B.
Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing
title Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing
title_full Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing
title_fullStr Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing
title_full_unstemmed Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing
title_short Investigation of Recurrent Melioidosis in Lao People's Democratic Republic by Multilocus Sequence Typing
title_sort investigation of recurrent melioidosis in lao people's democratic republic by multilocus sequence typing
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889735/
https://www.ncbi.nlm.nih.gov/pubmed/27001759
http://dx.doi.org/10.4269/ajtmh.15-0909
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