Cargando…
Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoc...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889804/ https://www.ncbi.nlm.nih.gov/pubmed/27298822 http://dx.doi.org/10.1155/2016/4275718 |
_version_ | 1782435018944020480 |
---|---|
author | Li, Yang Wang, Yixin Fang, Lichun Fu, Jiayuan Cui, Shuai Zhao, Yingjie Cui, Zhizhong Chang, Shuang Zhao, Peng |
author_facet | Li, Yang Wang, Yixin Fang, Lichun Fu, Jiayuan Cui, Shuai Zhao, Yingjie Cui, Zhizhong Chang, Shuang Zhao, Peng |
author_sort | Li, Yang |
collection | PubMed |
description | The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successfully identified. Three overlapping genomic fragments were obtained by PCR amplification and sequencing. The full genome sequence of the SD1403 strain was obtained by aligning the sequences. The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains. The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved. Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics. In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV. |
format | Online Article Text |
id | pubmed-4889804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-48898042016-06-13 Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China Li, Yang Wang, Yixin Fang, Lichun Fu, Jiayuan Cui, Shuai Zhao, Yingjie Cui, Zhizhong Chang, Shuang Zhao, Peng Biomed Res Int Research Article The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successfully identified. Three overlapping genomic fragments were obtained by PCR amplification and sequencing. The full genome sequence of the SD1403 strain was obtained by aligning the sequences. The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains. The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved. Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics. In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV. Hindawi Publishing Corporation 2016 2016-05-19 /pmc/articles/PMC4889804/ /pubmed/27298822 http://dx.doi.org/10.1155/2016/4275718 Text en Copyright © 2016 Yang Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Yang Wang, Yixin Fang, Lichun Fu, Jiayuan Cui, Shuai Zhao, Yingjie Cui, Zhizhong Chang, Shuang Zhao, Peng Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China |
title | Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China |
title_full | Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China |
title_fullStr | Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China |
title_full_unstemmed | Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China |
title_short | Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China |
title_sort | genomic analysis of the chicken infectious anemia virus in a specific pathogen-free chicken population in china |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889804/ https://www.ncbi.nlm.nih.gov/pubmed/27298822 http://dx.doi.org/10.1155/2016/4275718 |
work_keys_str_mv | AT liyang genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT wangyixin genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT fanglichun genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT fujiayuan genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT cuishuai genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT zhaoyingjie genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT cuizhizhong genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT changshuang genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina AT zhaopeng genomicanalysisofthechickeninfectiousanemiavirusinaspecificpathogenfreechickenpopulationinchina |