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Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China

The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoc...

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Autores principales: Li, Yang, Wang, Yixin, Fang, Lichun, Fu, Jiayuan, Cui, Shuai, Zhao, Yingjie, Cui, Zhizhong, Chang, Shuang, Zhao, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889804/
https://www.ncbi.nlm.nih.gov/pubmed/27298822
http://dx.doi.org/10.1155/2016/4275718
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author Li, Yang
Wang, Yixin
Fang, Lichun
Fu, Jiayuan
Cui, Shuai
Zhao, Yingjie
Cui, Zhizhong
Chang, Shuang
Zhao, Peng
author_facet Li, Yang
Wang, Yixin
Fang, Lichun
Fu, Jiayuan
Cui, Shuai
Zhao, Yingjie
Cui, Zhizhong
Chang, Shuang
Zhao, Peng
author_sort Li, Yang
collection PubMed
description The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successfully identified. Three overlapping genomic fragments were obtained by PCR amplification and sequencing. The full genome sequence of the SD1403 strain was obtained by aligning the sequences. The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains. The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved. Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics. In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV.
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spelling pubmed-48898042016-06-13 Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China Li, Yang Wang, Yixin Fang, Lichun Fu, Jiayuan Cui, Shuai Zhao, Yingjie Cui, Zhizhong Chang, Shuang Zhao, Peng Biomed Res Int Research Article The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV. In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification. A CIAV strain (SD1403) was isolated and successfully identified. Three overlapping genomic fragments were obtained by PCR amplification and sequencing. The full genome sequence of the SD1403 strain was obtained by aligning the sequences. The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains. The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved. Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics. In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV. Hindawi Publishing Corporation 2016 2016-05-19 /pmc/articles/PMC4889804/ /pubmed/27298822 http://dx.doi.org/10.1155/2016/4275718 Text en Copyright © 2016 Yang Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Yang
Wang, Yixin
Fang, Lichun
Fu, Jiayuan
Cui, Shuai
Zhao, Yingjie
Cui, Zhizhong
Chang, Shuang
Zhao, Peng
Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
title Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
title_full Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
title_fullStr Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
title_full_unstemmed Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
title_short Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
title_sort genomic analysis of the chicken infectious anemia virus in a specific pathogen-free chicken population in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4889804/
https://www.ncbi.nlm.nih.gov/pubmed/27298822
http://dx.doi.org/10.1155/2016/4275718
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