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The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms
BACKGROUND: Implant infections are a major complication in the field of orthopaedics. Bacteria attach to the implant-surface and form biofilm-colonies which makes them difficult to treat. Not only immune cells exclusively respond to bacterial challenges, but also local tissue cells are capable of pa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890488/ https://www.ncbi.nlm.nih.gov/pubmed/27250617 http://dx.doi.org/10.1186/s12891-016-1091-y |
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author | Dapunt, Ulrike Giese, Thomas Stegmaier, Sabine Moghaddam, Arash Hänsch, Gertrud Maria |
author_facet | Dapunt, Ulrike Giese, Thomas Stegmaier, Sabine Moghaddam, Arash Hänsch, Gertrud Maria |
author_sort | Dapunt, Ulrike |
collection | PubMed |
description | BACKGROUND: Implant infections are a major complication in the field of orthopaedics. Bacteria attach to the implant-surface and form biofilm-colonies which makes them difficult to treat. Not only immune cells exclusively respond to bacterial challenges, but also local tissue cells are capable of participating in defense mechanisms. The aim of this study was to evaluate the role of osteoblasts in the context of implant infections. METHODS: Primary osteoblasts were cultivated and stimulated with free-swimming bacteria at 4 °C and 37 °C. Supernatants were harvested for ELISA and expression of pro-inflammatory cytokines evaluated by RT-PCR. Bacterial binding to osteoblasts was evaluated using cytofluorometry and uptake was investigated by (3)H thymidine-labelling of bacteria. Osteoblasts were additionally stimulated with the extracellular polymeric substance (EPS) of Staphylococcus epidermidis biofilms, as well as components of the EPS; the bacterial heat shock protein GroEL in particular. RESULTS: We demonstrated that binding of bacteria to the osteoblast cell surface leads to an increased production of pro-inflammatory cytokines. Bacteria are capable of surviving intracellular. Furthermore, osteoblasts do not only respond to free-swimming, planktonic bacteria, but also to components of the EPS, including lipoteichoic acid and the heat shock protein GroEL. CONCLUSION: In conclusion, local tissue cells, specifically osteoblasts, might contribute to the persistence of the inflammatory response associated with implant-infections. |
format | Online Article Text |
id | pubmed-4890488 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-48904882016-06-03 The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms Dapunt, Ulrike Giese, Thomas Stegmaier, Sabine Moghaddam, Arash Hänsch, Gertrud Maria BMC Musculoskelet Disord Research Article BACKGROUND: Implant infections are a major complication in the field of orthopaedics. Bacteria attach to the implant-surface and form biofilm-colonies which makes them difficult to treat. Not only immune cells exclusively respond to bacterial challenges, but also local tissue cells are capable of participating in defense mechanisms. The aim of this study was to evaluate the role of osteoblasts in the context of implant infections. METHODS: Primary osteoblasts were cultivated and stimulated with free-swimming bacteria at 4 °C and 37 °C. Supernatants were harvested for ELISA and expression of pro-inflammatory cytokines evaluated by RT-PCR. Bacterial binding to osteoblasts was evaluated using cytofluorometry and uptake was investigated by (3)H thymidine-labelling of bacteria. Osteoblasts were additionally stimulated with the extracellular polymeric substance (EPS) of Staphylococcus epidermidis biofilms, as well as components of the EPS; the bacterial heat shock protein GroEL in particular. RESULTS: We demonstrated that binding of bacteria to the osteoblast cell surface leads to an increased production of pro-inflammatory cytokines. Bacteria are capable of surviving intracellular. Furthermore, osteoblasts do not only respond to free-swimming, planktonic bacteria, but also to components of the EPS, including lipoteichoic acid and the heat shock protein GroEL. CONCLUSION: In conclusion, local tissue cells, specifically osteoblasts, might contribute to the persistence of the inflammatory response associated with implant-infections. BioMed Central 2016-06-02 /pmc/articles/PMC4890488/ /pubmed/27250617 http://dx.doi.org/10.1186/s12891-016-1091-y Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Dapunt, Ulrike Giese, Thomas Stegmaier, Sabine Moghaddam, Arash Hänsch, Gertrud Maria The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
title | The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
title_full | The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
title_fullStr | The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
title_full_unstemmed | The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
title_short | The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
title_sort | osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890488/ https://www.ncbi.nlm.nih.gov/pubmed/27250617 http://dx.doi.org/10.1186/s12891-016-1091-y |
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