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Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism
Some endoparasitoid wasps lay eggs that produce cells called teratocytes. In this study, we sequenced and analyzed the transcriptome of teratocytes from the solitary endoparasitoid Cotesia vestalis (Braconidae), which parasitizes larval stage Plutella xylostella (Plutellidae). Results identified man...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890588/ https://www.ncbi.nlm.nih.gov/pubmed/27254821 http://dx.doi.org/10.1038/srep26967 |
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author | Gao, Fei Gu, Qi-juan Pan, Jing Wang, Ze-hua Yin, Chuan-lin Li, Fei Song, Qi-sheng Strand, Michael R. Chen, Xue-xin Shi, Min |
author_facet | Gao, Fei Gu, Qi-juan Pan, Jing Wang, Ze-hua Yin, Chuan-lin Li, Fei Song, Qi-sheng Strand, Michael R. Chen, Xue-xin Shi, Min |
author_sort | Gao, Fei |
collection | PubMed |
description | Some endoparasitoid wasps lay eggs that produce cells called teratocytes. In this study, we sequenced and analyzed the transcriptome of teratocytes from the solitary endoparasitoid Cotesia vestalis (Braconidae), which parasitizes larval stage Plutella xylostella (Plutellidae). Results identified many teratocyte transcripts with potential functions in affecting host immune defenses, growth or metabolism. Characterization of teratocyte-secreted venom-like protein 8 (TSVP-8) indicated it inhibits melanization of host hemolymph in vitro, while two predicted anti-microbial peptides (CvT-def 1 and 3) inhibited the growth of bacteria. Results also showed the parasitized hosts lacking teratocytes experienced higher mortality after immune challenge by pathogens than hosts with teratocytes. Taken together, these findings indicate that C. vestalis teratocytes secrete products that alter host immune functions while also producing anti-microbial peptides with functions that help protect the host from infection by other organisms. |
format | Online Article Text |
id | pubmed-4890588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-48905882016-06-09 Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism Gao, Fei Gu, Qi-juan Pan, Jing Wang, Ze-hua Yin, Chuan-lin Li, Fei Song, Qi-sheng Strand, Michael R. Chen, Xue-xin Shi, Min Sci Rep Article Some endoparasitoid wasps lay eggs that produce cells called teratocytes. In this study, we sequenced and analyzed the transcriptome of teratocytes from the solitary endoparasitoid Cotesia vestalis (Braconidae), which parasitizes larval stage Plutella xylostella (Plutellidae). Results identified many teratocyte transcripts with potential functions in affecting host immune defenses, growth or metabolism. Characterization of teratocyte-secreted venom-like protein 8 (TSVP-8) indicated it inhibits melanization of host hemolymph in vitro, while two predicted anti-microbial peptides (CvT-def 1 and 3) inhibited the growth of bacteria. Results also showed the parasitized hosts lacking teratocytes experienced higher mortality after immune challenge by pathogens than hosts with teratocytes. Taken together, these findings indicate that C. vestalis teratocytes secrete products that alter host immune functions while also producing anti-microbial peptides with functions that help protect the host from infection by other organisms. Nature Publishing Group 2016-06-02 /pmc/articles/PMC4890588/ /pubmed/27254821 http://dx.doi.org/10.1038/srep26967 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Gao, Fei Gu, Qi-juan Pan, Jing Wang, Ze-hua Yin, Chuan-lin Li, Fei Song, Qi-sheng Strand, Michael R. Chen, Xue-xin Shi, Min Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
title | Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
title_full | Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
title_fullStr | Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
title_full_unstemmed | Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
title_short | Cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
title_sort | cotesia vestalis teratocytes express a diversity of genes and exhibit novel immune functions in parasitism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890588/ https://www.ncbi.nlm.nih.gov/pubmed/27254821 http://dx.doi.org/10.1038/srep26967 |
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