Concentric-Flow Electrokinetic Injector Enables Serial Crystallography of Ribosome and Photosystem-II

In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-t...

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Detalles Bibliográficos
Autores principales: Sierra, Raymond G., Gati, Cornelius, Laksmono, Hartawan, Dao, E. Han, Gul, Sheraz, Fuller, Franklin, Kern, Jan, Chatterjee, Ruchira, Ibrahim, Mohamed, Brewster, Aaron S., Young, Iris D., Michels-Clark, Tara, Aquila, Andrew, Liang, Mengning, Hunter, Mark S., Koglin, Jason E., Boutet, Sébastien, Junco, Elia A., Hayes, Brandon, Bogan, Michael J., Hampton, Christina Y., Puglisi, Elisabetta V., Sauter, Nicholas K., Stan, Claudiu A., Zouni, Athina, Yano, Junko, Yachandra, Vittal K., Soltis, S. Michael, Puglisi, Joseph D., DeMirci, Hasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4890631/
https://www.ncbi.nlm.nih.gov/pubmed/26619013
http://dx.doi.org/10.1038/nmeth.3667
Descripción
Sumario:In this work, a concentric-flow electrokinetic injector delivered microcrystals of Geobacillus stearothermophilus thermolysin (2.2 Å structure), Thermosynechococcus elongatus photosystem II (< 3 Å diffraction) and Thermus thermophilus small ribosomal subunit (3.4 Å structure). The first ambient-temperature X-ray crystal structure of the 30S subunit bound to the antibiotic paromomycin was obtained in its native mother liquor. Compared to previous cryo-cooled structures, this new structure showed that paromomycin binds to the decoding center in a different conformation.

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